Grafted photo-polymerized monolithic column

Details Grafted photo-polymerized monolithic column Grafted photo-polymerized monolithic column

2006-02-28

2010-10-19

Therkorn, Ernest G (Department: 1797)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C536S023100

Reexamination Certificate

active

07815802

ABSTRACT:
The present invention relates to the fabrication of a grafted, UV photo-polymerized silica-based monolithic column and the use of such column for the extraction of DNA. In one embodiment, a method is provided for fabricating a silica-based monolithic column, wherein a vessel is filled with a polymerization mixture that is formed into monolithic solid phase for DNA extraction through in situ photo-polymerization.

REFERENCES:
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patent: 6887384 (2005-05-01), Frechet et al.
patent: 2003/0062308 (2003-04-01), Zare et al.
patent: 2003/0062310 (2003-04-01), Zare et al.
patent: 2006/0078983 (2006-04-01), Lau
patent: 2007/0284308 (2007-12-01), Zare et al.
Wen, et al., “DNA Extraction Using a Tetramethyl Orthosilicate-Grafted Photopolymerized Monolithic Solid Phase,” Analytical Chemistry, 2006, pp. 1673-1681, vol. 78, No. 5, American Chemical Society, Washington, DC, USA.

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