GPIb.alpha. fragments and recombinant DNA expression vectors

Details GPIb.alpha. fragments and recombinant DNA expression vectors GPIb.alpha. fragments and recombinant DNA expression vectors

1990-11-14

1994-08-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 698, 435 703, 4351723, 4352402, 4353201, 536 235, 530381, 514 8, 514822, 935 11, 935 27, 935 36, 935 56, 935 70, 935 71, C12N 1512, C12N 1585, A61K 3702

Patent

active

053407270

ABSTRACT:
Recombinant DNA expression vectors encoding a peptide which inhibits binding of von Willebrand factor to platelet membrane glycoprotein Ib, said vector including a nucleotide sequence encoding the amino acid sequence from HIS.sup.1 to LEU.sup.610, inclusive, of the amino terminal region of platelet membrane glycoprotein Ib.alpha., or any sequential subset thereof; mammalian host cells transformed by said vectors; and a process for producing a peptide having the identifying characteristics of the 45 kDa tryptic fragment of glycocalicin comprising the steps of (A) providing a stable, extrachromosomally replicable vector capable of directing in mammalian cells the expression of a nucleotide sequence encoding an amino acid sequence which includes said fragment, said nucleotide sequence further encoding as part of said amino acid sequence amino acids which are not native to said fragment, and which are oriented at the carboxy terminus of said fragment, (B) transforming said mammalian cells with said vector, and (C) maintaining said transformed mammalian cells under conditions permitting the expression of said peptide.

REFERENCES:
patent: 4664884 (1987-05-01), Hawiger et al.
patent: 4766075 (1988-08-01), Goeddel et al.
patent: 4880788 (1989-11-01), Moake et al.
Lopez, J. A. et al. "Cloning of the .alpha.-chain of human platelet . . . " Proc. Natl. Acad. Sci. 84: 5615-5619 (Aug. 1987).
Handa, M. et al., "The von Willebrand Factor-binding Domain . . . " J. Biol. Chem. 261(27):12579-12585 (Sep. 1986).
Michelson, A. D., et al., "Partial Characterization of a Binding . . . " Blood 67(1): 19-26 (Jan. 1986).
Glover, D. M., "Gene Cloning: The Mechanics of DNA Manipulation" pp. 179-218 (1984).

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