GOT isozyme assay

Details GOT isozyme assay GOT isozyme assay

1990-02-23

1992-06-16

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 15, 435 23, 435184, 435183, C12Q 148, C12Q 152, C12N 999

Patent

active

051224550

ABSTRACT:
A glutamic-oxaloacetic transaminase (GOT) isozyme assay, which comprises selectively inhibiting s-GOT in a sample such as serum or plasma by proteinase K, optionally an excess amount of proteinase K by a protease inhibitor and then determining m-GOT remaining intact, is described.

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Jany, K.-D., and Mayer, B. Biol. Chem. Hoppe-Seyler 366:485-492, May 1985.
Ebeling, W. et al., Eur. J. Biochem. 47, 91-97 (1974).
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Panteghini, M., et al. Clinical Chemistry 33, 67-71 (1987).
Lebherz, H. G., et al. Biological Abstracts vol. 81, No. 11, 1986, Abstract No. 101161.
Teranishi, H., et al, Clinical Biochemistry, vol. 21, 347-358 (1988).
Aoyagi, T., et al, Chemical Abstracts, vol. 85, No. 1, p. 145, 85:1676s, Jul. 1976.

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