Plant husbandry – Process
Patent
1990-02-12
1993-02-23
Fox, David T.
Plant husbandry
Process
435 691, 435 697, 435 698, 435 701, 4351723, 435183, 800205, 800255, 800DIG14, 800DIG17, 800DIG24, 800DIG26, 800DIG27, 800DIG43, 800DIG44, 935 35, 935 48, 935 67, 935 64, 504206, 504205, 504195, 504197, 504117, A01H 100, A01H 510, C12P 2100, C12P 2104, C12N 1500, C12N 900, A01N 5700
Patent
active
051886421
ABSTRACT:
This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.
The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
REFERENCES:
patent: 4940835 (1990-07-01), Shah et al.
Fraley Robert T.
Horsch Robert B.
Rogers Stephen G.
Shah Dilip M.
Fox David T.
Hoerner, Jr. Dennis R.
Monsanto Company
Stanley Howard C.
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