Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1986-07-07
1990-07-10
Warren, Charles F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351723, 4352404, 435320, 536 27, 935 35, 935 47, 935 48, 935 64, 935 67, 800DIG44, 800DIG43, 800DIG26, 800DIG14, 800DIG17, 800DIG63, 800DIG27, 800DIG24, 800230, 800DIG67, A01H 104, C12N 1500, C12N 500, C07H 1512
Patent
active
049408356
ABSTRACT:
This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.
The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
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Fraley Robert T.
Horsch Robert B.
Rogers Stephen G.
Shah Dilip M.
Fox David T.
Hoerner, Jr. Dennis R.
Monsanto Company
Swaney Larry R.
Warren Charles F.
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