Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-07-18
1998-08-25
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435193, C12P 1918, C12N 910
Patent
active
057982333
ABSTRACT:
The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc .beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.l.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1. fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).
REFERENCES:
patent: 5180674 (1993-01-01), Roth
Sandlin and Stein, 1994, J. Bacteriol. 176:2930-37.
Zhou et al., 1994, J. Biol. Chem. 269:11162-69.
Gibson et al., 1993, J. Bacteriol. 175:2702-12.
Jennings et al., 1993, Molec. Microbiol. 19:361-69.
Robertson et al., 1993, Molec. Microbiol. 8:891-901.
van Putten, 1993, EMBO J. 12:4043-51.
Verheul et al., 1993, Microbiol. Rev. 57:34-49.
Yamasaki et al., 1993, J. Bacteriol. 175:4565-68.
Kerwood et al., 1992, Biochemistry 31:12760-68.
Knight et al., 1992, Molec. Microbiol. 6:1565-73.
Mandrell, 1992, Infect. Immunol. 60:3017-20.
Pradel et al., 1992, J. Bacteriol. 174:4736-45.
John et al., 1991, J. Biol. Chem. 266:19303-11.
Jonsson et al., 1991, EMBO J. 10:477-88.
Mandrell et al., 1991, J. Bacteriol. 173:2823-32.
Schneider et al., 1991, J. Exp. Med. 174:1601-05.
Tsai and Civin, 1991, Infect. Immun. 59:3604-09.
Mandrell et al., 1990, J. Exp. Med. 171:1649-64.
Parsons et al., 1989, Microb. Pathog. 7:63-72.
Dudas and Apicella, 1988, Infect. Immun. 56:499-504.
Mandrell et al., 1988, J. Exp. Med. 168:107-26.
Schneider et al., 1988, Infect. Immun. 56:942-46.
Apicella et al., 1987, Infect. Immun. 55:1755-61.
Webster et al., 1983, J. Biol. Chem. 258:10637-41.
Danaher et al. (1995) Genetic Basis of Neisseria gonorrhoeae Lipooligosaccharide Antigenic Variation. J. Bact. 177(24): 7275-7279.
Cope et al. (1991) Molecular Cloning of a Gene Involved in Lipooligosaccharide Biosynthesis and Virulence Expression by Haemophilus influenzae Type B. Mol. Microbiol. 5(5): 1113-1124.
High et al. (1993) The Role of a Repetitive DNA Motif (5'-CAAT-3') in the Variable Expression of the Haemophilus influenzae Lipopolysaccharide Epitope Alpha-Gal(1,4)Beta-Gal. Mol. Microbiol. 9(6): 1275-1282.
Zhou et al. (1994) Lipooligosaccharide Biosynthesis in Neisseria gonorrhoeae: Cloning, Identification and Characterization of the Alpha 1,5 Heptosyltransferase I Gene (rfaC). Mol. Microbiol. 14(4): 609-618.
Hobbs Lisa J.
The Rockefeller University
Wax Robert A.
LandOfFree
Glycosyltransferases for biosynthesis of oligosaccharides, and g does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Glycosyltransferases for biosynthesis of oligosaccharides, and g, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glycosyltransferases for biosynthesis of oligosaccharides, and g will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-35523