Glycosyltransferase, nucleic acid encoding the...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S053000, C435S069100, C435S320100, C435S193000, C536S023200, C536S023500, C530S350000

Reexamination Certificate

active

07396666

ABSTRACT:
A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a biological sample as being cancerous when the transcription level of the nucleic acid in the biological sample significantly exceeds that in a normal biological sample as a control. The present invention also relates to a β1,3-N-acetyl-D-glucosaminyltransferase protein having an activity of transferring N-acetyl-D-glucosamine from a donor substrate to an acceptor substrate through β1,3-linkage.

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Strausberg et al. “Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences” Proc. Nat. Acad. Sci. USA 99:16899-16903 (2002).
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Ishida et al. “A novel β1,3-N-acetylglucosaminyltransferase (β3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer” FEBS Letters 579:71-78 (2005).
Vavasseur et al., Synthesis of O-glycan core 3: characterization of UDP-GlcNAc: GalNAc-R beta 3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines,Glycobiology, 1995, vol. 5, No. 3, pp. 351-357.
Vavasseur et al.,Synthesis of O-glycan core 3: characterization of UDP-GlcNAc: GalNAc-R beta 3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines, Glycobiology, 1995, vol. 5, No. 3, pp. 351-357.

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