Glycosyltransferase genes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C536S023200, C435S320100, C435S252330, C435S254200, C435S069200, C435S015000, C435S252300, C435S325000, C435S193000

Reexamination Certificate

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10048866

ABSTRACT:
A nucleic acid encoding a protein which has an amino acid sequence according to SEQ ID NO: 2, 12, or 14, or the amino acid sequence modified by addition or deletion of one or more amino acids and/or substitution by other amino acids, and which has activity to transfer a glycosyl group to the 3′-position of anthocyanins.

REFERENCES:
patent: 967283 (1999-12-01), None
patent: WO 99/05287 (1999-02-01), None
Fukuchi-Mizutani, M. et al., “Biochemical and Molecular Characterization of a Novel UDP-Glucose:Anthocyanin 3′-O-Glucosyltransferase, a Key Enzyme for Blue Anthocyanin Biosynthesis, from Gentian”,Plant Physiology 132(3): 1652-1663, (2003).
Winkel-Shirley, B. et al., “Flavonoid Biosynthesis. A Colorful Model for Genetics, Biochemistry, Cell Biology, and Biotechnology”,Plant Physiology 126(2): 485-493, (2001).
Markham, K. R., “Novel Anthocyanins Produced in Petals of Genetically Transformed Lisianthus”, Phytochemistry 42(4): 1035-1038, (1996).
Horvath, D. M., et al., “Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds”,Plant Molecular Biology 31: 1061-1072, (1996).
Yoshida, K., et al., “Contribution of each caffeoyl residue of the pigment molecule of gentiodephin to blue color development”,Phytochemistry 54: 85-92, (2000).
Tanaka, Y., et al., “Molecular and Biochemical Characterization of Three Anthocyanin Synthetic Enzymes fromGentiana triflora”, Plant Cell Physiol, 37(5): 711-716, (1996).
Mato, Masami, et al., “Isolation and Characterization of a cDNA Clone of UDP-Galactose: Flavonoid 3-O-Galactosyltransferase (UF3GaT) Expresed inVigna mungoSeedlings”,Plant Cell Physiol. 39(11): 1145-1155, (1998).
Miller, K. D., et al., “Purification, Cloning, and Heterologous Expression of a Catalytically Efficient Flavonal 3-O-Galactosyltransferase Expressed in the Male Gametophyte ofPetunia hybrida”, The Journal of Biological Chemistry 274(48): 34011-34019, (1999).

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