Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Patent
1995-06-07
1998-03-17
Wax, Robert A.
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
424 946, 424 9461, 435183, A61K 3846, A61K 3847, C12N 900
Patent
active
057283818
ABSTRACT:
The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
REFERENCES:
patent: 4866042 (1989-09-01), Neuwelt
Wilson, et al. (1990) Hunter Syndrome: Isolation of an Iduronate-2-Sulfatase cDNA and Analysis of Patient DNA. Proc. Natl. Acad. Sci. USA 87:8531-8535.
Neufeld, et al. (1989) in The Metabolic Basis of Inherited Disease, entitled "The Mucopolysaccharidoses", eds Scriver, CR, Beauden, AL, Sly, WS & Valle, D (McGraw-Hill), New York pp. 1565-1587.
Shapiro, (1989) in The Metabolic Basis of Inherited Disease, entitled "Steroid Sulfatase Deficiency and Z-linked Ichthyosis", eds Scriver, CR, Beauden, AL, Sly, WS & Valle, D (McGraw-Hill, New York) pp. 1945-1964.
Bielicki, et al. (1990) "Human liver iduronate-2-sulphatase" Biochem J 271: pp. 75-86.
Nelson, et al. (1989) "Cystic fibrosis:prenatal diagnosis and carrier detection by DNA analysis" Med J Aust 151:pp. 126-131.
Stein, et al. (1989) "Cloning and Expression of Human Arylsulfatase A*" J Biol Chem 264(2):pp. 1252-1259.
Peters, et al. (1990) "Phylogenetic Conservation of Arylsulfatases" J Biol Chem 265(6):pp. 3374-3381.
Yen, et al. (1988) "The Human X-Linlked Steroid Sulfatase Gene and a Y-Encoded Pseudogene:Evidence for an Inversion of the Y Chromosome during Primate Evolution" Cell 55:pp. 1123-1135.
Robertson, et al. (1988) "Human Glucosamine-6-sulfatase cDNA Reveals Homology with Steroid Sulfatase"Biochem Biophys Res Commun 157(1) pp.: 218-224.
Yen, et al. (1987) "Cloning and Expression of Steroid Sulfatase cDNA and the Frequent Occurrence of Deletions in STS Deficiency:Implications for X-Y Interchange" Cell 49:pp.443-454.
Stein, et al. (1989) "Cloning and Expression of Human Steroid-sulfatase" J Biol Chem 264(23):pp. 13865-13872.
Sasaki, et al. (1988) "cDNA cloning, nucleotide sequence and expression of the gene for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) embryo" Eur J Biochem 177: pp. 9-13.
Anson, et al. (1992) "Correction of human mucopolysaccharidosis type VI fibroblast with recombinant N-acetygalactosamine-4-sulphatase" Biochem J 284:pp. 289-794.
Bielicki et al. (1993) "Recombinant Human Iduronate-2-Sulphatse: Correction of Mucopolysacchoridosis-Type II Fibtroblasts and Characterization of the Purific Enzyme" Biochem. J. 289: 241-246.
Gottesman, (1987) "Chinese Hamster Ovary Cells" Meth. Enzymol. 157: 3-8.
American Type Culture Collector Catalog of Cell Lines and Hybridomas, Seventh Edition, 1992, p. 166.
Darnell et al. (1986) in Molecular Cell Biology, entitled "Protein Glycosylation" (Scientific American Books, New York, New York) pp. 957-964.
Ferrara et al., (1987) "Characterization of Recombinant Glycosylated Human Interleutin-2 Produced by a Recombinant Plasma Transformed CHO Cell Line" FEBS. Lett. 266(1): 47-52.
Kodama et al., (1992) "Characterization of Recombinant Murine Interleution 5 Express in Chinese Hamster Ovary Cells" Glycobiol. 2(5): 419-427.
Eliahu, R., Am J. Hum. Genet 33:576-583 (1981).
Anson Donald Stewart
Bielicki Julie
Clements Peter Roy
Hopwood John Joseph
Morris Charles Phillip
Stole Einar
Wax Robert A.
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