Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2008-03-18
2008-03-18
Kemmerer, Elizabeth C. (Department: 1646)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C536S023100, C536S023500, C530S350000, C530S351000, C514S002600, C514S008100, C514S012200
Reexamination Certificate
active
10651395
ABSTRACT:
Disclosed are human G-CSF isoforms having a modified amino acid sequence containing a glycosylation sequence Asn-X-Ser/Thr (N-X-S/T) at one or more specific amino acid positions according to the present invention, genes encoding the human G-CSF isoforms, and expression vectors carrying the genes, eukaryotic cells transformed or transfected with the expression vectors. Also, the present invention discloses a method of preparing a glycosylated human G-CSF isoform, comprising the steps of culturing the transformant or transfectant and isolating a glycosylated human G-CSF isoform from the culture supernatant or cell lysates, a human G-CSF isoform prepared by the method, and a pharmaceutical composition comprising the human G-CSF isoform.
REFERENCES:
patent: 5217881 (1993-06-01), Park
patent: 5298395 (1994-03-01), Park
patent: 5756349 (1998-05-01), Lin
patent: 2004/0254351 (2004-12-01), Beals et al.
Hübel et al., 2003, Ann. Hematol. 82 :207-213.
Basu et al., 2002, Int. J. Mol. Med. 10:3-10.
Takashi Kuwabara, et al.,Pharmacokinetics and Pharmacodynamics of a Recombinant Human Granulocyte Colony-Stimulating Factor, Drug Metabolism Reviews, 28(4), pp. 625-658, (1996).
Masayoshi Oh-Eda, et al.,O-Linked Sugar Chain of Human Granulocyte Colony-stimulating Factor Protects It Against Polymerization and Denaturation Allowing It to Retain Its Biological Activity, The Journal of Biological Chemistry, vol. 265, No. 20, Jul. 15, 1990, pp. 11432-11435.
Brian I. Lord, et al.,Kinetics of Neutrophil Production in Normal and Neutropenic Animals During the Response to Filgrastim(r-metHu G-CSF)or Filgrastim SD/01(PEG-r-metHu G-CSF), Clinical Cancer Research, vol. 7, Jul. 2001, pp. 2085-2090.
Graham Molineaux, et al.,A New Form or Filgrastim with Sustained Duration in Vivo and Enhanced Ability to Mobilize PBPC in Both Mice and Humans, Experimental Hematology 27 (1999), pp. 1724-1734.
Belinda R. Avalos,Molecular Analysis of the Granulocyte Colony-Stimulating Factor Receptor, The Journal of The American Society of Hematology, vol. 88, No. 3, Aug. 1, 1996, pp. 761-777.
Judith E. Layton, et al.,Identification of Ligand-Binding Site III on the Immunoglobulin-Like Domain of the Granulocyte Colony-Stimulating Factor Receptor, The Journal of Biological Chemistry, vol. 276, No. 39, Sep. 28, 2001, pp. 36779-36787.
Osamu Hiraoka, et al.,Requirement for the Immunoglobulin-Like Domain of Granulocyte Colony-Stimulating Factor Receptor in Formation of a 2:1 Receptor-Ligand Complex, The Journal of Biological Chemistry, vol. 270, No. 43, Oct. 27, 1995, pp. 25928-25934.
Judith E. Layton, et al.,Interaction of Granulocyte Colony-Stimulating Factor(G-CSF)With Its Receptor, The Journal of Biological Chemistry, vol. 274, No. 25, Jun. 18, 1999, pp. 17445-17451.
Chung Jong Sang
Kim Hyun Seok
Kim Ki Wan
Kim Yeon Hyang
Koh Hyung Kon
CJ Corp
Hunton & Williams
Kemmerer Elizabeth C.
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