Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1993-01-19
1994-11-01
Hill, Jr., Robert J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530397, 435 691, 435 695, 435 694, 4351721, C07K 1300
Patent
active
053608968
ABSTRACT:
Disclosed are (1) a mutein of a fibroblast growth factor (FGF), the DNA having introduced therein at least one nucleotide sequence coding for a glycosylation site, (2) a DNA coding for the mutein of (1), (3) a vector containing the DNA of (2), (4) a transformant transformed with the vector of (3), and (5) a process for producing the mutein which comprises cultivating in a culture medium the transformant of a yeast or animal cell transformed with a vector of (3), and producing and accumulating the mutein of (1) in the culture medium, whereby the FGF mutein into which at least one glycosylation site has been introduced is improved in productivity, stability and activities, and advantageously used as medicine.
REFERENCES:
Jambarini et al. J. of Biol. Chem 257 (16): 9692-97 (1982).
Abraham et al. Science 233: 545-548 (1986).
Biochem, Hoppe-Seyler, vol. 368, No. 9 (1987), p. 1060, B. Hofer et al., "Modification and Introduction of Protein Glyxcosylation Sites by Site-Specific Mutagenesis:Studies on Human Interleukin 2".
Biochemical and Biophysical Research Communications, vol. 138, No. 2 (1986) pp. 611-617, G. Gimenez-Gallego et al., "The Complete Amino Acid Sequence of Human Brain-Derived Acidic Fibroblast Growth Factor".
Biochemical and Biophysical Research Communications, vol. 151, No. 2 (1988), pp. 701-708, H. Seno et al., "Stabalizing Basic Fibroblast Growth Using Protein Engineering".
The Journal of Biological Chemistry, vol. 263, (1988), pp. 18452-18458, G. Fox et al., "Production, Biological Activity, and Structure of Recombinant Basic Fibroblast Growth Factor and an Analog with Cysteine Replaced by Serine".
FEBS Letters, vol. 213, (1987), pp. 189-194, T. Kurokawa et al., "Cloning and expression of cDNA encoding human basic fibroblast growth factor".
Igarashi Koichi
Sasada Reiko
Senoo Masaharu
Buckley Linda M.
Cermak Shelly Guest
Conlin David G.
Hill Jr. Robert J.
Takeda Chemical Industries Ltd.
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