Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1994-07-21
1995-11-07
Robinson, Douglas W.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
436501, 536 551, 536 552, 536 53, 5361231, 435 724, 435 71, G01N 33564
Patent
active
054647789
ABSTRACT:
P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [.sup.3 H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
REFERENCES:
patent: 4783330 (1988-11-01), Furie et al.
patent: 5198424 (1993-03-01), McEver
Aruffo, A., et al., "CD62/P-Selectin Recognition Of Myeloid and Tumor Cell Sulfatides", Cell, 67: 35-44 (1991).
Aruffo, A., et al., "Granule Membrane Protein 140 (CMP140) Binds To Carcinomas And Carcinoma-Derived Cell Lines", Proc. Natl. Acad. Sci. USA, 89: 2292-2296 (1992).
Beckstead, J. H., et al., "Immunohistochemical Localization of Membrane and .alpha.-Granule Proteins in Human Megakaryocytes: Application to Plastic-Embedded Bone Marrow Biopsy Specimens", Blood, 67: 285-293 (1986).
Bevilacqua, M. P., et al., "Identification of an Inducible Endothelial-leukocyte Adhesion Molecule", Proc. Natl. Acad. Sci. USA, 84:9238-9242 (1987).
Bevilacqua, M. P., et al., "Endothelial Leukocyte Adhesion Molecule 1: An Inducible Receptor for Neutophils Related to Complement Regulatory Proteins and Lectins", Science, 243:1160-1165 (1989).
Bienvenu, K. and N. Granger, "Molecular Determinants of Shear Rate-Dependent Leukocyte Adhesion in Postcapillary Venules", Am. J. Physiol., 264 (Heart Circ. Physiol., 33): H1504-H1508 (1993).
Bonfanti, R., et al., "PADGEM (GMP140) is a Component of Weibel-Palade Bodies of Human Endothelial Cells," Blood, 73(5): 1109-1112 (1989).
Borman, S., "Glycotechnology Drugs Begin To Emerge From The Lab", Chem. Eng. News, pp. 27-34 (Jun. 28, 1993).
Brandley, B. K., et al., "Carbohydrate Ligands of the LEC Cell Adhesion Molecules", Cell, 63:861-863 (1990).
Buttrum, S. M., et al., "Selectin-Mediated Rolling of Neutrophils On Immobilized Platelets", Blood, 82: 1165-1174 (1993).
Corral, L., et al., "Requirement For Sialic Acid On Neutrophils In A GMP-140 (PADGEM) Mediated Adhesive Interaction With Activated Platelets", Biochem. Biophys. Res. Comm., 172(3): 1349-1356 (1990).
Dore, M., et al., "P-Selectin Mediates Spontaneous Leukocyte Rolling in Vivo", Blood, 82: 1308-1316 (1993).
Dunlop, L. C., et al., "Characterization Of GMP-140 (P-Selectin) As A Circulating Plasma Protein", J. Exp. Med., 175: 1147-1150 (1992).
Gamble, J. R., et al., "Prevention of Activated Neutrophil Adhesion to Endothelium by Soluble Adhesion Protein GMP140", Science, 249: 414-417 (1990).
Geng, J-G., et al., "Lectin Domain Peptides From Selectins Interact With Both Cell Surface Ligands and Ca.sup.2+ Ions", J. Biol. Chem., 267: 19846-19853 (1992).
Geng, J-G., et al., "Rapid Neutrophil Adhesion To Activated Endothelium Mediated By GMP-140", Nature, 343:757-760 (1990).
Grober, J. S., et al., "Monocyte-Endothelial Adhesion In Chronic Rheumatoid Arthritis", J. Clin. Invest., 91: 2609-2619 (1993).
Hamburger and McEver, "GMP-140 Mediates Adhesion of Stimulated Platelets to Neutrophils," Blood, 75: 550-554 (1990).
Handa, K., et al., "Selectin GMP-140 (CD62; PADGEM) Binds To Sialosyl-Le.sup.a And Sialosyl-Le.sup.x, And Sulfated Glycans Modulate This Binding", Biochem. Biophys. Res. Commun., 181: 1223-1230 (1991).
Hattori, R., et al., "Complement Proteins C5b-9 Induce Secretion Of High Molecular Weight Multimers Of Endothelial Von Willebrand Factor And Translocation Of Granule Membrane Protein GMP-140 To The Cell Surface", J. Biol. Chem., 264(15): 9053-9060 (1989).
Hattori, R., et al., "Stimulated Secretion Of Endothelial Von Willebrand Factor Is Accompanied By Rapid Redistribution To The Cell Surface Of The Intracellular Granule Membrane Protein GMP-140", J. Biol. Chem., 264(14): 7768-7771 (1989).
Hoff, S. D., et al., "Increased Expression Of Sialyl-Dimeric Le.sup.x Antigen In Liver Metastases Of Human Colorectal Carcinoma", Cancer Res., 49: 6883-6888 (1989).
Johnston, et al., "Cloning Of GMP-140, A Granule Membrane Protein Of Platelets And Endothelium: Sequence Similarity To Proteins Involved In Cell Adhesion And Inflammation", Cell, 56: 1033-1044 (1989).
Johnston, et al., "Structural And Biosynthetic Studies Of The Granule Membrane Protein, GMP-140, From Human Platelets And Endothelial Cells", J. Biol. Chem., 264: 1816-1823 (1989).
Johnston, G. I., et al., "Cloning of GMP-140: Chromosomal Localization, Molecular Heterogeneity And Identification Of cDNAs Predicting Both Membrane Bound And Soluble Proteins", Blood, 72, Suppl.: 327a, Abstract No. 1218 (1988).
Johnston, G. I. et al., "Structure of Human Gene Encoding Granule Membrane Protein-140 of the Selectin Family of Adhesion Receptors for Leukocytes", J. Biol. Chem., 265(34): 21381-21385 (1990).
Johnston, et al., "Structure and Biosynthesis Of The Platelet .alpha.-Granule Membrane Protein, GMP-140 ", Blood, 70(5), Suppl. 1: 352a Abstract No. 1264 (1987).
Kijima-Suda, I., et al., "Possible Mechanism Of Inhibition Of Experimental Pulmonary Metastasis of Mouse Colon Adenocarinoma 26 Sublines By A Sialic Acid: Nucleoside Conjugate", Cancer Res., 48: 3728-3732 (1988).
Kojima, N., et al., "Inhibition Of Selectin-Dependent Tumor Cell Adhesion To Endothelial Cells And Platelets By Blocking O-Clycosylation Of These Cells", Biochem. Biophys. Res. Commun., 182: 1288-1295 (1992).
Larsen, et al., "PADGEM Protein: A Receptor That Mediates The Interaction Of Activated Platelets With Neutrophils And Monocytes", Cell, 59: 305-312 (1989).
Larsen, E., et al., "PADGEM-Dependent Adhesion Of Platelets To Monocytes And Neutrophils Is Mediated By A Lineage-Specific Carbohydrate, LNF III (CD15)", Cell, 63: 467-474 (1990) (GMP140 carbohydrate ligand).
Levinovitz, A., et al., "Identification Of A Clycoprotein Ligand For E-Selectin On Mouse Myeloid Cells", J. Cell Biol. 121:449-459 (1993).
Liao, L., et al., "Oxidized Lipoproteins Elcit Leukocyte-Endothlial Cell Adhesion in Mesenteric Venules", FASEB J. (Abstracts), 7:abstract no. 1986 (1993).
Lowe, J. B., et al., "A Transfected Human Fucosyltransferase cDNA Determines Biosynthesis Of Oligosaccharide Ligand(s) For Endothelial-Leukocyte Adhesion Molecule", Biochem. Soc. Trans., 19: 549-653 (1991).
Mayadas, T. N., et al., "Leukocyte Rolling And Extravasation Are Severely Compromised in P Selectin-Deficient Mice", Cell, 74: 541-554 (1993).
McEver, R. P., "Misguided Leukocyte Adhesion", J. Clin. Invest., 91: 2340-2341 (1993).
McEver, R. P., "Selectins: Novel Receptors that Mediate Leukocyte Adhesion During Inflammation", Thrombosis and Haemostasis, F. K. Schattauer Verlagsgesellschaft mbH (Stuttgart), 65(3):223-228 (1991).
McEver and Martin, "A Monoclonal Antibody to a Membrane Glycoprotein Binds Only to Activated Platelents", J. Biol. Chem., 259: 9799-9804 (1984).
McEver, R. P., "GMP-140: A Receptor For Neotrophils And Monocytes On Activated Platelets And Endothelium", J. Cell. Biochem., 45: 156-161 (1991).
McEver, R. P., "leukocyte-Endothelial Cell Interactions", Curr. Opin. Cell Biol., 4: 840-849 (1992).
McEver, R., et al., "GMP-140, A Platelet .alpha.-Granule Membrane Protein, Is Also Synthesized By Vascular Endothelial Cells And Is Localized In Weibel-Palade Bodies", J. Clin. Invest., 84: 92-99 (1989).
McEver, et al., "The Platelet .alpha.-Granule Membrane Protein GMP-140 Is Also Synthesized By Human Vascular Endothelial Cells and Is Present In Blood Vessels of Diverse Tissues", Blood, 70(5) Suppl. 1: 355a, Abstract No. 1274 (1987).
McEver, R. P. et al., "Properties Of GMP-140, An Inducible Granule Membrane Protein Of Platelets And Endothelium", Blood Cells, 16: 73-83 (1990).
Moore, K. L., et al., "Identification Of A Specific Glycoprotein Ligand For P-Selectin (CD62) On Myeloid Cells", J. Cell Biol., 118: 445-456 (1992).
Moore, K. L. and L. F. Thompson, "P-Selectin (CD62) Binds To Subpopulation Of Human Memory T Lymphocytes And N
Cummings Richard D.
McEver Rodger P.
Moore Kevin L.
Board of Regents of the University of Oklahoma
Pabst Patrea L.
Robinson Douglas W.
Varma Anita
LandOfFree
Glycoprotein ligand for P-selectin and methods of use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Glycoprotein ligand for P-selectin and methods of use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glycoprotein ligand for P-selectin and methods of use thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-196909