Glycoprotein and process for producing the same

Details Glycoprotein and process for producing the same Glycoprotein and process for producing the same

2002-06-14

2009-08-25

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S006120, C435S071100

Reexamination Certificate

active

07579166

ABSTRACT:
A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with α-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.

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Yasunori Chiba et al., “Production in yeast of α-galactosidase-A, a lysosomal enzyme applicable to enzyme replacement therapy for Fabry disease”, Glycobiology, vol. 12, No. 12, pp. 821-828, 2002.
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JP 10-155495 A (Director General, Agency of Industrial Science and Technology) Jun. 16, 1998.
R. Schiffman, et al., Infusion of α-galactosidase A reduces tissue globotriaosylceramide storage in patients with Fabry disease. Proc. Natl. Acad. Sci. USA, Jan. 2000, vol. 97, No. 1, pp. 365-370.

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