Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1982-08-13
1986-07-08
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435 68, 435 70, 435256, 435317, 935 11, 935 12, 935 28, 935 37, 935 6, C12P 2104, C12P 2102, C12N 118, C12N 100
Patent
active
045993114
ABSTRACT:
Promoters associated with expression of specific enzymes in the glycolytic pathway are used for expression of alien DNA, particularly yeast promoters known to provide high enzyme levels of enzymes in the glycolytic pathway are employed for expressing a mammalian protein, such as .alpha..sub.1 -antitrypsin. The promoters include promoters involved in expression of pyruvate kinase, triose phosphate isomerase, phosphoglucose isomerase, phosphoglycerate mutase, hexokinase 1, hexokinase 2, glucokinase, phosphofructokinase, and aldolase, as well as the glycolytic regulation gene. Particularly, the glycolytic regulation gene can be used in conjuction with promoters in the glycolytic pathway for regulated production of desired proteins.
REFERENCES:
Hitzeman et al, The Journal of Biological Chemistry, vol. 255, No. 24, pp. 12073-12080, Dec. 25, 1980.
Tuite et al, The EMBO Journal, vol. 1, No. 5, pp. 603-608 (1982).
Hitzeman et al, Nature, vol. 293, pp. 717-722, Oct. 29, 1981.
Strathern et al, The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression, pp. 1-37, 590, 599 & 604 (1982).
Woo et al, Miami Winter Symposia, vol. 19, From Gene to Protein: Translation Into Biotechnology, p. 579 (Jan. 1982).
Dobson et al, Nucleic Acids Research, vol. 10, No. 8, pp. 2625-2637, Apr. 24, 1982.
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