Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1994-09-02
1997-01-28
Knode, Marian C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436530, 436 87, 436811, 436828, 435975, G01N 33553, G01N 33544, G01N 3300
Patent
active
055977359
ABSTRACT:
A Dot-Blot assay ("spot test") with Bis-N,N,dioctadecylamide (BDA.TDA) as antigen was developed to detect anti-BDA.TDA antibodies in tuberculosis patients. To develop the antigen-antibody reaction, as a first step and in order to enhance the reaction, an anti-human rabbit serum was used followed by incubation with a protein A-colloidal gold conjugate. This assay showed almost equal sensitivity and specificity as the .beta.-galactosidase ELISA test which was conducted in parallel. This simple and fast assay could be used in places where ELISA equipment is not easily available.
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Handzel Vera
Laszlo Adalbert
Vera-Cabrera Lucio
Anderson J. Wayne
Duffy Patricia A.
Her Majesty the Queen in right of Canada as represented by the
Knode Marian C.
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