Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Having -c- – wherein x is chalcogen – bonded directly to...
Reexamination Certificate
2000-06-20
2001-12-04
Seaman, D. Margaret (Department: 1625)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Having -c-, wherein x is chalcogen, bonded directly to...
C514S424000, C514S613000, C514S616000, C514S626000, C549S517000, C549S557000, C564S102000, C564S153000, C564S159000, C564S152000, C564S192000, C564S197000, C564S199000
Reexamination Certificate
active
06326393
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to analogs that display selective inhibitory activity against the aspartyl proteases, plasmepsin and cathepsin D.
BACKGROUND OF THE INVENTION
Resistance to known antimalarial therapies is becoming an increasing problem and new therapies are therefore desperately needed. Upon infecting a host, the malaria parasite avidly consumes the host hemoglobin as its source of nutrients. Plasmepsin I and II are proteases from Plasmodium falciparum that are necessary during the initial stages of hemoglobin hydrolysis and digestion, which occurs in the &agr;-chain, between Phe 33 and Leu 34, then other sites serve as substrates for hydrolysis as well. In culture, inhibition of plasmepsin by a peptidomimetic inhibitor is demonstrated as effective in preventing malarial hemoglobin degradation and in killing the parasite (Francis, S. E., Gluzman, I. Y., Oksman, A., Knickerbocker, A., Mueller, R., Bryant, M. L., Sherman, D. R., Russell, D. G., and Goldberg, D. E. (1994)
EMBO J
, 13, 306-317). Thus, persons of skill in the art expect that plasmepsin inhibitors will provide effective antimalarial therapy in humans.
Cathepsin D is a human protease in the endosomal-lysosomal pathway, involved in lysosomal biogenesis and protein targeting, and may also be involved in antigen processing and presentation of peptide fragments. The protease therefore displays broad substate specificity, but prefers hydrophobic residues on either side of the scissile bond.
Cathepsin D has been implicated in a variety of diseases, including connective tissue disease, muscular dystrophy, and breast cancer. Cathepsin D is also believed to be the protease which processes the &bgr;-amyloid precursor protein (Dreyer, R. N., Bausch, K. M., Fracasso, P., Hammond, L. J., Wunderlich, D., Wirak, D. O., Davis, G., Brini, C. M., Bucholz, T. M., Konig, G., Kamarck, M. E., and Tamburini, P. P. (1994)
Eur. J. Biochem
., 224, 265-271 and Ladror, U. S., Synder, S. W., Wan, G. T., Holzman, T. F., and Krafft, G. A. (1994)
J. Biol. Chem
., 269, 18422-18428), generating the major component of plaques in the brains of Alzheimer's patients. Consequently, persons of skill in the art expect that inhibitors of cathepsin D will be useful in treating Alzheimer's disease.
The present invention relates to peptidornimetic (hydroxystatine amides and hydroxyphosphonates) analogs and their inhibitory action against aspartyl proteases. More particularly, the invention relates to the identification of such compounds that display selective inhibitory activity against plasmepsin and cathepsin D. Although statine-containing peptides are known which inhibit aspartyl proteases (Shewale, J. G.; Takahashi, R.; Tang, J., Aspartic Proteinases and Their Inhibitors, Kostka, V., Ed. Walter de Gruyter: Berlin (1986) pp. 101-116; U.S. Ser. No. 08/743,944, filed Nov. 5, 1996, which is hereby incorporated by reference in its entirety), there are only a few selective inhibitors for cathepsin D (Lin, T.-Y.; Williams, H. R., Inhibition of Cathepsin D by Synthetic Oligopeptides,
J. Biol. Chem
. (1979), 254, 11875-11883; Rich, D. H.; Agarwal, N. S., Inhibition of Cathepsin D by Substrate Analogues Containing Statine and by Analogues of Pepstatin,
J. Med. Chem. (
1986) 29 (2519-2524)), and for plasmepsin (Silva, A. M. et al., Srctzure and Inhibition of Plasmepsin II, A Hemoglobin-Degrading Enzyme From Plasmodizim falciparum,
Proceed Natl Acad Sci
, 1996, 93, 10034-10039).
The present invention also relates to the solid phase synthesis of such peptidomimetic analogs.
SUMMARY OF THE INVENTION
I. Preferred Embodiments
The compounds of the present invention are represented by Formula I:
wherein:
R
1
is chosen from the group consisting of alkyl, —(CH
2
)
n
-cycloalkyl, —(CH
2
CH
2
)
n
NHC(O)-alkyl, and arylalkyl, wherein n=1-3;
R
2
is H or
where
is a solid support, and -L- is a linker;
Y is —P(O)(OR
3
)
2
or —CH(OH)C(O)TNR
4
R
5
, wherein R
3
is alkyl, arylakyl, or haloalkyl; and R
4
and R
5
are independently chosen from the group consisting of H, alkyl, —(CH
2
)
n
-cycloalkyl, —(CH
2
CH
2
)
n
NHC(O)-alkyl, arylalkyl,
—C(H)(R
9
)CH
2
OR
10
, —C(H)(R
11
)C(H)(OR
10
)(R
11
), -alkyl-NHSO
2
R
11
, —C(H)(R
9
)C(O)NHR
10
, and —C(H)(R
9
)C(O)NHC(H)(R
9
)C(O)NHR
10
, wherein n=1-3;
R
9
is independently selected from the group consisting of alkyl and arylalkyl;
R
10
is independently selected from the group consisting of H, alkyl, and arylalkyl;
R
11
is independently selected from the group consisting of alkyl and aryl; or, when taken together, R
4
and R
5
can be
wherein R
12
and R
13
are independently selected from the group consisting of H, halo, and alkoxy; and
Z is —C(O)R
6
and —C(O)C(H)(R
7
)OC(O)NHR
8
, wherein R
6
is alkyl, arylalkyl, aryl, —(CH
2
)
m
-cycloalkyl, heteroaryl, or
wherein m=0-3;
R
7
is H, alkyl, arylalkyl, or —(CH
2
)
n
-cycloalkyl; and
R
8
is alkyl, arylalkyl, or aryl.
Preferred compounds of Formula I are those wherein -L- is of Formula (a)
wherein the designated meta-position is attached to the —C(O)—and the orthomethylene attaches to the amide nitrogen of Formula I.
A preferred embodiment of the invention are compounds of Formula I wherein:
Y is —P(O)(OR
3
)
2
, wherein R
3
is arylalkyl; and
Z is
Another preferred embodiment of the invention are compounds of Formula I wherein:
Y is —C(H)(OH)C(O)NHR
5
, wherein R
5
is
wherein R
9
is independently selected from the group consisting of alkyl and arylalkyl; and
Z is
wherein R
8
is alkyl or arylalkyl.
A further preferred embodiment of the invention are compounds of Formula I wherein:
Y is —C(H)(OH)C(O)NHR
5
, wherein R
5
is —C(H)(R
11
)C(H)(OR
10
)(R
11
), wherein
R
11
is aryl and R
10
is H, wherein each R
11
may be the same or different; and
Z is
wherein R
8
is alkyl or arylalkyl.
Yet another preferred embodiment of the invention are compounds of Formula I wherein:
Y is —C(H)(OH)C(O)NER
5
, wherein R
5
is —C(H)(R
11
)C(H)(OR
10
)(R
11
), wherein
R
11
is aryl and R
10
is H, wherein each R
11
may be the same or different; and
Z is R
6
C(O)—, wherein R
6
is alkyl, arylalkyl, —(CH
2
)
m
-cycloalkyl, or
Another aspect of the invention is the use of divinylbenzene-cross-linked, polyethylenelycol-grafted polystyrene beads optionally functionalized with amino groups (e.g., TentaGel™ S NH
2
, Rapp Polymere) as the solid supports for constructing, compounds of Formula I.
REFERENCES:
Evans, CA 117:251667, 1992.*
Evans, CA 116:174631, 1992.*
Dellaria, CA 110:39369, 1987.*
Sham, CA 105:227324, 1986.
Carroll Carolyn DiIanni
Dolle, III Roland Ellwood
Herpin Timothee Felix
Shimshock Yvonne Class
Heslin Rothenberg Farley & & Mesiti P.C.
Pharmacopeia Inc.
Seaman D. Margaret
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