Glycoconjugated fluorescent labeling reagents

Details Glycoconjugated fluorescent labeling reagents Glycoconjugated fluorescent labeling reagents

1997-04-25

1999-03-02

Higel, Floyd D.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

530802, 536 65, 435 78, 548455, C07H 2102, C07H 1708, O07H 2104, G01N 3353

Patent

active

058773100

ABSTRACT:
Water soluble glycoconjugated fluorescent labeling reagents comprised of a fluorescent dye, one or more sugar or carbohydrate residues that impart water solubility, and a reactive group that allows covalent attachment of the reagent to a substrate are disclosed. Glycoconjugated fluorescent labeling reagents are depicted as follows: ##STR1## These glycoconjugates are highly water soluble because of the sugar residues. This property is expected to lower nonspecific binding to cellular matter, reduce precipitation of labeled substrates, inhibit quenching of fluorescence, and unlike many prior fluorescent labeling reagents, that are soluble only in organic solvents, render the glycoconjugates usable in wholly aqueous solution.

REFERENCES:
patent: 4259233 (1981-03-01), Carrico et al.
patent: 4621911 (1986-11-01), Lanni et al.
patent: 4933948 (1990-06-01), Herkstroeter
patent: 4966607 (1990-10-01), Shinoki et al.
patent: 4981977 (1991-01-01), Southwick et al.
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5316906 (1994-05-01), Haugland et al.
patent: 5486616 (1996-01-01), Waggoner et al.
Bannwarth et al, "Formation of Carboxamides with N,N,N',N'-Tetramethyl *Succinimido) Uromium Tetrafluoroborate in Aqueous / Organic Solvent Systems", Tetrahedron Letters, vol. 32, No. 9, pp.1157-1160 (1991).
Singh et al, "Controlled Coupling of Aminoglycoside Antibiotics to Prietins for use in Homogeneous Enzyme Innumoassays",Can. J. Chem., vol.62 (1984),pp. 2471-2477.
Whitaker et al, "Cascade Blue Derivatives: Water Soluble, Reactive, Blue Emisison Dyes Evaluated as Fluorescent Labels and Tracers",Analytical Biochemistry198, 119-130 (1991).
Derwent Publication, XP002077545, 30 Oct. 1992, "Water Soluble Derivative Preparation React Derivative Beta Cyclodextrin Carbozymethyl Beta Cyclodextrin Dissolve Product Water Boiling Filter"(1 page).
Reddington, "New Glycoconjugated Cyanine Dyes as Fluorescent Labeling Reagents", J. Chem. Soc. Perkin Trans. 1.No. 1. 1998, pp. 143-147.
Guether et al, "Photostable Cyanine Dye .beta.-Cyclodextrin Conjugates",Tetrahedon Letters, vol. 38, No. 35, pp.6167-6170 (1997).
J. Chem. Soc. Faraday Trans 1994, 90(23) 3517-3520 Matsuzawa et al Light Stability of a .beta.-cyclodextrin.dagger.Inclusion Complex of a Cyanine Dye.
Research News Adv Mater 1993, 5, No. 2 Akihiko Ueno Fluorescent Sensores and Color-Change Indicators for Molecules.

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