Glycerol kinase, which has high resistance against preservative

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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Reexamination Certificate

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07618800

ABSTRACT:
The disclosure relates to (i) a gene isolated fromCellulomonassp.JCM2471, the gene encoding a new glycerol kinase and (ii) a method for preparing the glycerol kinase by gene recombination technique, A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant vector, and a method for producing the glycerol kinase, including culturing the transformant to produce glycerol kinase, and collecting the resulting glycerol kinase.

REFERENCES:
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Bradshaw et al., N-Terminal processing: the methionine aminopeptidase and N-acetyl transferase families., TIBS, 1998, vol. 23, pp. 263-267.
Cole et al., (1998) “Deciphering the biology ofMicrobacterium tuberculosisfrom the complete genome sequence.”, Nature 393:537-544.
Cole et al., (1998) “Deciphering the biology ofMicrobacterium tuberculosisfrom the complete genome sequence.”, Nature 393:537-544 (Version with corrections ).
Huang et al., “Cloning, Sequencing, High Expression , and Crystallization of the ThermophileTermus aquaticusGlycerol Kinase,” Biosci. Biotechnol. Biochem. 62(12):2375-2381, 1998.

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