Glycerol-3-phosphate o-acyltransferase promoter for gene...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

Reexamination Certificate

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C435S041000, C435S045000, C435S071100

Reexamination Certificate

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11225354

ABSTRACT:
The promoter region associated with theYarrowia lipolyticaglycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.

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NCBI Accession CR382129, Dujon, B. Genome evolution in yeasts. Nature. Jul. 1, 2004;430(6995):35-44 (see attached search print out titled SCORE Search Result Application 11225354 and Search Result us-11-225-354-17.rge ).
Dujon, B. Genome evolution in yeasts. Nature. Jul. 1, 2004;430(6995):35-44.
U.S. Appl. No. 11/183,664, filed Jul. 18, 2005, Picataggio et al.
Colin Ratledge, Microbial Oils and Fats: An Assessment of Their Commercial Potential, Progress in Industrial Microbiology, vol. 16:119-206, 1982.
Madzak, Catherine et al., Heterologous protein expression and secretion in the non-conventional yeastYarrowia lipolytica: a review, Journal of Biotechnology, 2004, 109, 63-81, Elsevier B.V.

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