Glutathione s-transferase

Details Glutathione s-transferase Glutathione s-transferase

1996-11-26

1998-10-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

43525233, 4352523, 4353201, 536 231, 536 232, 536 235, C12N 910, C12N 120

Patent

active

058174974

ABSTRACT:
The present invention provides a human glutathione S-transferase (HGST) and polynucleotides which identify and encode HGST. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding HGST and a method for producing HGST. The invention also provides for agonists, antibodies, or antagonists specifically binding HGST, and their use, in the prevention and treatment of cancer and other diseases associated with the expression of HGST. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HGST for the treatment of cancer and other diseases associated with the expression of HGST. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding HGST.

REFERENCES:
Lee, HC et al., "A molecular genetic approach for the identification of essential residues in human glutathione S-transferase function in Escherichia coli" J. Biol. Chem. 270 (1) :99-109 (1995).
Stenberg, G et al., "Effects of directed mutagenesis on conserved arginine residues in a human Class Alpha glutathione transferase" Biochem. J.274:549-555 (1991).
Thier, R et al., "Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes" Proc. Natl. Acad. Sci. USA90:8567-8580 (1993).
Simula, TP et al., Human glutathione S-transferase-expressing Salmonella typhimurium tester strains to study the activation/detoxification of mutagenic compounds: studies with halogenated compounds, aromatic amines and aflatoxin B.sub.1 Carcinogenesis14(7):1371-1376 (1993).
Dirven, HAAM et al., "Involvement of Human Glutathione S-Transferase Isoenzymes in the Conjugation of Cyclophosphamide Metabolites with Glutathione" Cancer Res.54:6215-6220 (1994).
Klone, A et al., "Cloning, sequencing and characterization of the human Alpha glutathione S-transferase gene corresponding to the cDNA clone pGTH2" Biochem. J.285:925-928 (1992) (Accession GI 825605).
Stenberg, G et al., "Heterologous expression of recombinant human glutathione transferase A1-1 from a hepatoma cell line" Protein Expr Purif3:80-84 (1992) (Accession GI 259141).
Zimniak, P et al., "A subgroup of class .alpha. glutathione S-transferases Cloning of cDNA for mouse lung glutathione S-transferase GST 5.7" FEBS Lett313(2):173-176 (1992) (Accession GI 193710).

Affiliated with

Also associated with

Rating

Glutathione s-transferase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glutathione s-transferase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glutathione s-transferase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-76083