Glutathione peroxidase gene and process for production of the ge

Details Glutathione peroxidase gene and process for production of the ge Glutathione peroxidase gene and process for production of the ge

1989-06-15

1992-02-18

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

4352523, 4352401, 536 27, C12N 1553, C12N 908, C12N 1500

Patent

active

050894080

ABSTRACT:
DNA which encodes at least a polypeptide containing glutathione peroxidase activity and having an amino acid sequence from the N-terminal to the C-terminal of the formula: ##STR1## wherein * * * is selenocystein, is produced by culturing transformant host cells which possess extraneous DNA encoding a polypeptide with glutathione peroxidase activity in a medium containing selenium, and separating a thus-produced polypeptide with glutathione peroxidase activity from the cultured mass.

REFERENCES:
Mullenbach et al., NAR, vol. 15, p. 5484, 1987.
Sukenaga et al., NAR, vol. 15, p. 7178, 1987.
Chambers et al., EMBO, vol. 5, pp. 1221-1227, 1986.
Takahashi et al., Archives of Biochem. & Biophys., vol. 256, pp. 677-686, 1987.
"Primary Structure of Human Plasma Glutathione Peroxidase Deduced from cDNA Sequences", Journal of Biochemistry, vol. 108, 1990, by K. Takahashi et al., pp. 145-148.

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