Glutaraldehyde/polyethylenimine immobilization of whole microbia

Details Glutaraldehyde/polyethylenimine immobilization of whole microbia Glutaraldehyde/polyethylenimine immobilization of whole microbia

1981-03-30

1982-10-19

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435174, 435177, 435233, 435234, C12P 1924, C12N 1100, C12N 990, C12N 992

Patent

active

043551050

ABSTRACT:
Whole microbial cells containing enzymes not sensitive to glutaraldehyde such as sucrose mutase or glucose isomerase are immobilized by forming a reaction product of the cells in an aqueous medium with glutaraldehyde, reacting the reaction product with polyethylenimine to flocculate the reaction product, and recovering the flocculated reaction product from the aqueous medium. Reacting the cells with glutaraldehyde prior to reacting with polyethylenimine results in flocculated cells that can be more easily separated from the aqueous medium.

REFERENCES:
patent: 3779869 (1973-12-01), Zienty
patent: 3989597 (1976-11-01), Lee et al.
patent: 4212943 (1980-07-01), Borglum
patent: 4288552 (1981-09-01), Gestrelius

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