Glutaminase, its gene and a method of producing it

Details Glutaminase, its gene and a method of producing it Glutaminase, its gene and a method of producing it

2007-01-02

2007-01-02

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S228000

Reexamination Certificate

active

10851337

ABSTRACT:
Glutaminase is purified from anAspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene fromAspergillus oryzaegenomic library and cDNA library, andAspergillus nidulansgenomic library by hybridization using the partial sequence as a probe.

REFERENCES:
Shaffer et al. An asperaginase of Aspergillus nidulans is subject to oxygen repression in addition to nitrogen metabolie repression. Mol. Gen. Genet 1988, 212, 337-341.
T. Yano, et al., “Purification and Properties of Glutaminase FromAspergillus Oryzae”, J. Ferment. Technol., (1988), 66, No. 2, pp. 137-143.
T. Yano, et al., Agricultural and Biological Chemistry, vol. 55, No. 2, XP-001057982, pp. 387-391, “Production and Localization of Enzymes on Soft Gel Cultivation”, 1991.
K. Koibuchi, et al., Applied Microbiology and Biotechnology, vol. 54, No. 1, XP-002191327, pp. 59-68, “Molecular Cloning and Characterization of a Gene Encoding Glutaminase FromAspergillus Oryzae”, Jul. 2000.
Takahiro Ohba, et al., Purification ofAspergillus oryzaesp. glutaminase and cloning its gene (part 1), Institute of Biology and Food, Feb. 2, 2004, 1 page, (with partial English translation).

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