Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-06-21
1998-06-30
Marx, Irene
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
4352533, 435874, C12N 902
Patent
active
057735859
ABSTRACT:
A novel glutamate dehydrogenase is derived from the genus Pseudomonas and is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, has an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP.
REFERENCES:
Chavez et al., An NAD-Specific Glutamate Dehydrogenase from Cyanobacteria, Federation of European Biochemical Societies, 1991, vol. 285, No. 1, pp. 35-38.
Winnacker et al., Purification and Properties of a NAD-Dependent Glutamate Dehydrogenase from Clostridium SB.sub.4, Biochimica et Biophysica Acta, 1970, vol. 212, pp. 225-242.
Johnson et al., Purification and Characterization of Glutamic Acid Dehydrogenase and .alpha.-Ketoglutaric Acid Reductase from Peptococcus aerogenes, Canadian J. Microbiol., 1972, vol. 18, pp. 881-892.
Tokushige et al., J. Biochem., 85, 1415-20, 1979.
Fruh et al., Arch. Microbiol., 1985, 141, 170-76.
Joannou et al., FEMS Microbiology letters, 90, 1992, 205-210.
Mulligan et al., App. & Envir. Microbiol., 1989, 55(11), 3016-19.
Bellion, Proceedings of the 4.sup.th Int'l. Symposium on Microbiol growth on 1 carbon Compounds, 1984, 170-173.
Hattori Shizuo
Hongo Noriyuki
Kawamura Yoshihisa
Teshima Shin'ichi
Yamamoto Kazumi
Marx Irene
Toyo Boseki Kabushiki Kaisha
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