Glucuronyl C5-epimerase, DNA encoding the same and uses thereof

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Isomerase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023200, C536S023400

Reexamination Certificate

active

07399626

ABSTRACT:
The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.

REFERENCES:
patent: 5939289 (1999-08-01), Ertesvåg et al.
patent: 6372477 (2002-04-01), Jørsboe et al.
patent: 6764844 (2004-07-01), Lindahl et al.
patent: 6887698 (2005-05-01), Jalkanen et al.
patent: 2003/0186414 (2003-10-01), Gilbert et al.
patent: WO 96/14425 (1996-05-01), None
patent: WO 98/48006 (1998-10-01), None
patent: WO 01/38507 (2001-05-01), None
Campbell, P., et al., “Biosynthesis of Heparin/Heparan Sulfate: Purification of the D-Glucuronyl C-5 Epimerase from Bovine Liver,”J. Biol. Chem. 269:26953-26958, The American Society of Biochemistry and Molecular Biology Inc. (1994).
Casu, B., et al., “Conformational flexibility: a new concept for explaining binding and biological properties of iduronic acid-containing glycosaminoglycans,”Trends Biochem. Sci. 13:221-225, Elsevier Science Ltd. (1988).
Crawford, B.E., et al., “Cloning, Golgi Localization, and Enzyme Activity of the Full-length Heparin/Heparan Sulfate-Glucuronic Acid C5-epimerase”,J. Biol. Chem. 276:21538-21543, The American Society for Biochemistry and Molecular Biology, Inc. (Jun. 2001).
Database EBI, D-glucuronyl C5-epimerase (EC 5.1.3.-) amino acid sequence (mouse), Accession No. Q9EPS3, Entry Date Mar. 2001.
Database EBI, D-glucuronyl C5-epimerase (EC 5.1.3.-) amino acid sequence (bovine), Accession No. 018756, Entry Date Jan. 1998.
Database EBI, D-glucuronyl C5-epimerase amino acid sequence (EC 5.1.3.-), Accession No. 094923, Entry Date May 1999.
Database Biosis Biosciences Information Service, Accession No. PREV200000122890, abstract for document NPL10, Hagner-McWhirter, A. et al., “Biosynthesis of heparin/heparan sulphate: Kinetic studies of the glucuronyl C5-epimerase with N-sulfated derivatives of theEscherichia coliK5 capsular polysaccharide as substrates,”Glycobiology 10:159-171, Oxford University Press (Feb. 2000).
Dialog File 351, Accession No. 2001-381292/200140, Derwent WPI English language abstract for WO 01/38507 (May 31, 2001).
Féthière, J., et al., “Crystal Structure of Chondroitin AC Lyase, a Representative of a family of Glycosaminoglycan Degrading Enzymes,”J. Mol. Biol. 288:635-647, Academic Press (May 1999).
Hagner-McWhirter, Å. et al., “Biosynthesis of heparin/heparan sulfate: kinetic studies of the glucuronyl C5-epimerase with N-sulfated derivatives of theEsherichia coliK5 capsular polysaccharide as substrates,”Glycobiology 10:159-171, Oxford University Press (Feb. 2000).
Hagner-McWhirter, Å. et al., “Biosynthesis of heparin/heparan sulphate: mechanism of epimerization of glucuronyl C-5,”Biochem J. 347:69-75, London Portland Press (Apr. 2000).
International Search Report for International Application No. PCT/FI01/01068, European Patent Office, Netherlands, mailed on Dec. 9, 2002.
Kelley, L.A., et al., “Enhanced Genome Annotation Using Structural Profiles in the Program 3D-PSSM,”J. Mol. Biol. 299:499-520, Academic Press (Jun. 2000).
Kjellén, L. and Lindahl, U., “Proteoglycans: Structures and Interactions,”Annu. Rev. Biochem. 60:443-475, Annual Reviews Inc. (1991).
Li, J.-P., et al., “Biosynthesis of Heparin/Heparan Sulfate. cDNA Cloning and Expression of D-Glucuronyl C5-Epimerase from Bovine Lung.,”J. Biol. Chem. 272:28158-28163, The American Society for Biochemistry and Molecular Biology, Inc. (1997).
Li, J.-P., et al. , “Characterization of the D-Glucuronyl C5-epimerase Involved in the Biosynthesis of Heparin and Heparan Sulfate”,J. Biol. Chem. 276:20069-20077, The American Society for Biochemistry and Molecular Biology, Inc. (Jun. 2001).
Li, J.-P., et al., Targeted disruption of a murine glucuronyl C5-epimerase gene results in heparan sulfate lacking L-iduronic acid and in neonatal lethality,J. Biol. Chem. 278:26363-28366, The American Society for Biochemistry and Molecular Biology, Inc. (Aug. 2003).
Malmström, A., et al., “Biosynthesis of Heparin. Partial Purification of the Uronosyl C-5 Epimerase,”J. Biol. Chem. 255:3878-3883, The American Society of Biochemistry and Molecular Biology Inc. (1980).
Nagase, T., et al. , “Prediction of the Cloning Sequences of Unidentified Human Genes. XII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro,”DNA Res. 5:355-364, Tokyo Kazusa DNA Research Institute and Universal Academy Press (1998).
NCBI Entrez, GenBank Report, Accession No. LO8483, Xue, F., et al., Entry Date 1993.
NCBI Entrez, GenBank Report, Accession No. P46555, Sulston, J., Entry Date 1994.
NCBI Entrez, GenBank Report, Accession No. AF043700, Wilson, R., et al., Entry Date 1998.
NCBI Entrez, GenBank Report, Accession No. AAF36018, TheC. elegansSequencing Consortium et al., Entry Date 1998.
NCBI Entrez, GenBank Report, Accession No. AAF57373, from Adams, M.D., et al., Entry Date Mar. 2000.
PCT Partial International Search Report for International Application No. PCT/FI01/01068, filed Dec. 7, 2001, European Patent Office, Netherlands, mailed on Aug. 1, 2002.
Ron, D., et al., “Expression of Biologically Active Recombinant Keratinocyte Growth Factor. Structure/Function Analysis of Amino-Terminal Truncation Mutants,”J. Biol. Chem. 268:2984-2988, The American Society of Biochemistry Molecular Biology Inc. (1993).
Salmivirta, M., et al., “Heparan sulfate: a piece of information,”FASEB J. 10:1270-1279, Federation of American Societies for Experimental Biology (1996).
Voet, D. and Voet, J.G.,Biochemistry, 2nd Edition, John Wiley and Sons, Inc., New York, NY, pp. 965-967 (1995).
Wilson, R., et al., “2.2 Mb of contiguous nucleotide sequence from chromosome III ofC. elegans,” Nature 368:32-38, Macmillan Magazines Limited (1994).
Xue, F. and Cooley, L., “Kelch Encodes a Component of Intercellular Bridges in Drosophila Egg Chambers,”Cell 72:681-693, Cell Press (1993).
Office Action for U.S. Appl. No. 10/005,647, inventors Jalkanen et al., filed Dec. 7, 2001, mailed on May 4, 2004.
Office Action for U.S. Appl. No. 10/835,179, Lindahl, U., filed Apr. 30, 2004, mailed on Jul. 24, 2007, with supplemental clarification mailed Jul. 24, 2007, and the claims from U.S. Appl. No. 10/835,179 corresponding thereto.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Glucuronyl C5-epimerase, DNA encoding the same and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glucuronyl C5-epimerase, DNA encoding the same and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glucuronyl C5-epimerase, DNA encoding the same and uses thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3966657

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.