Glucuronide repressors and uses thereof

Details Glucuronide repressors and uses thereof Glucuronide repressors and uses thereof

2006-02-14

2006-02-14

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S006120, C530S350000, C530S388210, C536S023400

Reexamination Certificate

active

06998229

ABSTRACT:
Clones containing a sequence encoding a glucuronide repressor are described. The nucleotide and amino acid sequences of a repressor (gusR) are presented. A glucuronide repressor is used to control expression of a transgene, detect glucuronides in a sample, and isolate glucuronides from a sample, among other uses.

REFERENCES:
patent: 5268463 (1993-12-01), Jefferson
patent: 5432081 (1995-07-01), Jefferson
Blanco C. et al. Cloning and endonuclease restriction analysis of uidA and uidR genes inE.coliK12: determination of transcription direction for the uidA gene. J. Bacteriol. 1982, vol. 149:587-594, Feb. 1982.
Blanco C. et al. Negative dominanat mutations of the uidR gene inE.coli: genetic proof for a cooperative regulation of uidA expression. Genetics. 1986, vol. 112: 173-182, Feb. 1988.
Artandi SE et al. TFE3 contains two activation domains, one acidic and the other proline rich, that synergistically activate transcription. Nucleic Acids Res. 1995, vol. 23(19): 3865-3871, Feb. 1982.
Jefferson R.A. Gen Bank Accession No. AAA68922, dated Jun. 27, 1995.
Blanco et al., “Cloning and Endonuclease Restriction Analysis of uidA and uidR Genes inEscherichia coliK-12: Determination of Transcription Direction for the uidA Gene,”Journal of Bacteriology 149(2):587-594, 1982.
Blanco, “Transcriptional and translational signals of the uidA gene inEscherichia coliK12,”Mol Gen Genet 208:490-498, 1987.
Blanco et al., “Negative Dominant Mutations Of The UIDR Gene InEscherichia Coli: Genetic Proof For A Cooperative Regulation Of UIDA Expression,”Genetics 112: 173-182, 1986.
Ritzenthaler et al., “Interchangeability of Repressors for the Control of the uxu and uid Operons inE. coliK12,”Mol Gen Genet 191:263-270, 1983.
Wilson and Jefferson, “The GusR repressor ofEscherichia Coli,” EMBL Database Entry Ecuidaa; Accession No. M14641(Version 8), 1995.

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