Glucose transporter vesicle aminopeptidase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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C12Q 137

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059687649

ABSTRACT:
An aminopeptidase which is a component of GLUT4-containing vesicles in the natural state, and which cleaves insulin. The claimed protein has a molecular weight of approximately 110 kD in its deglycosylated form. It includes the amino acid sequences Phe-Ala-Ala-Thr-Gln-Phe-Glu-Pro-Leu-Ala-Ala [SEQ ID NO: 1] and Ile-Leu-Gln-Asn-Gln-Ile-Gln-Gln-Gln-Thr-Arg-Thr-Asp-Glu-Gly-Xaa-Pro-Xaa-Me t [SEQ ID NO: 2], and reacts with antibodies produced against the peptide identified as [SEQ ID NO: 1]. Modulators of the activity of the aminopeptidase and a method for treating syndromes of insulin resistance, including diabetes, by administration of such a modulator are also claimed.

REFERENCES:
patent: 5225354 (1993-07-01), Knowles et al.
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Kandror et al. (1994A) J.Biol.Chem., 269(1), "Identification and Isolation of Glycoproteins That Translocate to the cell Surface from GLUT4-Enriched Vesicles in an Insulin-Dependent Fashion", pp. 138-142.
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