Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1991-12-02
1993-06-01
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 4, 435 25, 435174, 435182, 204403, 204412, 204418, 204817, G01N 2746, A61B 500, C12N 1104, C12N 1108
Patent
active
052158870
ABSTRACT:
Disclosed is a method of measuring glucose concentration of a sample solution using a glucose sensor having an ion-sensitive field-effect transistor which can detect pH change in the sample solution provided with a glucose oxidase-immobilized film formed on the ion-sensing section thereof, which comprise a step of measuring the output of the glucose sensor under a low level of dissolved oxygen in the enzyme-immobilized film; a step of measuring the output of the glucose sensor under a high level of dissolved oxygen in the enzyme-immobilized film; and a step of determining the glucose concentration of the sample solution from the difference between said two output values. The measuring system can be simplified and working efficiency can be improved since the present measuring method requires neither a buffer nor correct measuring of the sample solution or buffer.
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patent: 4894339 (1990-01-01), Hanazato et al.
patent: 4935105 (1990-06-01), Churchouse
Casa et al., "pH-Based Enzyme Potentiometric Sensors"; Analytical Chemistry, vol. 57, pp. 1917-1925, 1985.
Leary Louise N.
NEC Corporation
Wityshyn Michael G.
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