Chemistry: molecular biology and microbiology – Condition responsive control process
Patent
1994-03-10
1996-07-09
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Condition responsive control process
424 937, 435 721, 435 14, 435 30, 435 34, 435 35, 435 694, 435 703, 4352401, 4352402, 43524021, 436172, C12N 506, C12N 508, C12Q 154, C12P 2100
Patent
active
055344046
ABSTRACT:
A method of selecting cells with enhanced secretion of a secretory product is disclosed. The method comprises exposing a population of cells to a secretagogue to result in the secretion of a secretory product from the cells and selecting from the population, cells that exhibit increased amounts of intracellular free calcium when exposed to the secretagogue. The method enables the selection of correctly regulated .beta. cells that secrete appropriate amounts of insulin in response to varying glucose levels.
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Fiore Deborah M.
Hegre Orion D.
Knaack David
Laurance Megan E.
Cytotherapeutics, Inc.
Lilling Herbert J.
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