Glucose isomerization under expanded bed conditions

Textiles: fiber preparation

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195 63, 195 68, 195DIG11, 195115, C12D 1302

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active

041382900

ABSTRACT:
Expanded bed isomerization of glucose syrup to a glucose-fructose mixture having a fructose proportion exceeding about 40% of the glucose-fructose content by passing 30-55% w/w of glucose syrup through a cell-mass glucose isomerase preparation in the form of a bed of uniform particles of controlled size in the range of 150-2000 micrometers at a superficial syrup velocity of 0.5-15 meters an hour. Velocity and particle size are inversely related to create bed expansion within the range of 5-100%.
Bed life may be extended by substituting fresh catalyst for inactivated catalyst.
The isomerase should have an activity exceeding about 100 IGIC u/gm.
The isomerase should be weighted by incorporation therein of finely divided alumina particles.

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patent: 3960663 (1976-06-01), Tamura et al.
patent: 3980521 (1976-09-01), Amotz et al.
Emery et al., "Some Applications of Solid-Phase Enzymes in Biological Engineering", Birmingham Univ. Chem. Engineer, vol. 22, No. 2, (Summer 1971), pp. 37-41.
Barker et al., "Enzyme Reactors for Industry", Process Biochemistry, vol. 6, No. 10 (Oct. 1971), pp. 11-13.

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