Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Isomerase
Patent
1991-09-19
1993-11-30
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Isomerase
435842, 935 10, 935 14, C12N 992
Patent
active
052664759
ABSTRACT:
A genetically engineered glucose isomerase with improved affinity for D-glucose and the method of preparation of such a glucose isomerase are disclosed. The glucose isomerase is obtained by mutagenizing the gene of a naturally occurring glucose isomerase such that a smaller amino acid replaces a larger amino acid in the catalytic site. In an especially advantageous embodiment of the present invention, the Clostridium glucose isomerase sequence is mutated and the residue replaced with a smaller amino acid is either Trp.sub.139 or Val.sub.186.
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Schink and Zeikus, "Clostridium thermosulfurogenes sp. nov., a New Thermophile that Produces Elemental Sulphur from Thiosulphate," Journal of General Microbiology (1983), 129, 1149-1158.
Lee et al., "Catalytic Mechanism of Xylose (Glucose) Isomerase from Clostridium thermosulfurogenes," Journal of Biological Chemistry (1990), vol. 265, No. 31, pp. 19082-19090.
Bagdasarian Michael
Lee Chanyong
Meng Menghsiao
Zeikus J. Gregory
Bugalsky Gabriele E.
Michigan Biotechnology Institute
Wax Robert A.
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