Glucose isomerase mutants

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Isomerase

Reexamination Certificate

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C435S105000, C435S094000, C536S023200

Reexamination Certificate

active

07919300

ABSTRACT:
This invention describes a series of recombinantThermoanaerobacterium saccharolyticumglucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.

REFERENCES:
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patent: 5656497 (1997-08-01), Zeikus et al.
patent: WO-03/062387 (2003-07-01), None
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Liu et al., “Purification and Cloning of a Thermostable Xylose (Glucose) Isomerase with an Acidic pH Optimum formThermoanaerobacteriumStrain JW/SL-YS 489”.J. of Bacteriol., v.178(20): pp. 5938-5945, 1996.
Kaneko et al., “Characterization of Acid-Stable Glucose Isomerase fromStreptomycessp., and Development of Single-Step Process for High-Fructose”.Biosci. Biotechnol. Biochem., v.64: pp. 940-947, 2000.
Lee et al., “Genetic Organization, Sequence and Biochemical Characterization of Recombinant β-xylosidase fromThermoanaerobacterium saccharolyticumStrain B6A-RI”.J. of Gen. Micro. Bio., v.139: pp. 1127-1234, 1993.
Vieille et al., “Xylose Isomerases fromThermotoga”. Methods of Enzymol., v. 330: pp. 215-224, 2001.
Lee et al., “Cloning, Sequencing and Biochemical Characterization of Xylose Isomerase fromThermoanaerobacterium saccharolyticumStrain B6A-RI”.J. of Gen. Microbiol., v.139: pp. 1241-1243, 1993.
Scriprapundh et al., “Molecular Determinants of Xylose Isomerase Thermal Stability and Activity: Analysis of Thermozymes by Site-Directed Mutagenesis”.Protein Engineering, v. 13: pp. 259-265, 2000.
Scriprapundh et al., “Directed Evolution ofThermatoga neapolitanaXylose Isomerase: High Activity on Glucose at Low Temperature and Low pH”.Protein Engineering, v.16: pp.683-690, 2003.
Lee et al., “Catalytic Mechanism of Xylose (Glucose) Isomerase fromClostridium thermosulfurogenes.” The Journal of Biological Chemistry, v.265:31, pp. 19082-19090 (1990) .
Liu et al., “Purification and Cloning of a Thermostable Xylose (Glucose) Isomerase with an Acidic pH Optimum fromThermoanaerobacteriumStrain JW/SL-YS 489.”Journal of Bacteriology, v.178:20, pp. 5938-5945 (1996).
Meng et al., “Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate.”Proc. Natl. Acad. Sci. USA, v.88, pp. 4015-4019 (1991).
Meng et al., “The role of active-site aromatic and polar residues in catalysis and substrate discrimination by xylose isomerase.”Proc. Natl. Acad. Sci. USA, v.90, pp. 8459-8463 (1993).
Lee et al., “Cloning, sequencing and biochemical characterization of yxlose isomerase fromThermoanaerobacterium saccharolyticumstrain B6A-RI.”Journal of General Microbiology, v.139, pp. 1227-1234 (1993).
Meaden et al., “The xylose isomerase-encoding gene (xylA) ofClostridium thermosaccharolyticum: cloning, sequencing and phylogeny of XylA enzymes.”Gene, v.141, pp. 97-101 (1994).

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