Glucose free, stable dry analytical element for quantitative ana

Details Glucose free, stable dry analytical element for quantitative ana Glucose free, stable dry analytical element for quantitative ana

1998-09-22

1999-12-07

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 74, 435188, C12Q 150

Patent

active

05998158&

ABSTRACT:
A dry analytical element for quantitatively analyzing creatine kinase MB isozyme is composed of a support and a creatine kinase MB isozyme-detecting agent layer. The detecting layer contains an antibody which specifically inhibits activity of M sub-unit of creatine kinase, creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase MB isozyme, a buffer compound which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound, said indicator composition having hexokinase, nicotinamide-adenine dinucleotide or its phosphate in an oxidized form, and glucose-6-phosphate dehydrogenase but not containing glucose in such amount as to give the spectroscopically detectable compound.

REFERENCES:
patent: 4067775 (1978-01-01), Wurzburg et al.
patent: 4080265 (1978-03-01), Antonik
patent: 4387160 (1983-06-01), Gomez et al.
patent: 4912033 (1990-03-01), Ladenson et al.
patent: 5298406 (1994-03-01), Loyd et al.
patent: 5804394 (1998-09-01), Suzuki et al.
Landt Y. Semi-automated Direct Colorimetric Measurement of Creatine Kinase Isoenzyme MB Activity After Extraction From Serum by Use of a CK-MB Specific Monoclonal Antibody, Clin Chem 34/3 575-581, 1988.

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