Glucose dehydrogenase β-subunit and DNA encoding the same

Details Glucose dehydrogenase β-subunit and DNA encoding the same Glucose dehydrogenase β-subunit and DNA encoding the same

2006-08-22

2006-08-22

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S069100, C435S325000, C435S320100, C435S242000, C435S252300, C435S252200, C435S190000, C536S023200

Reexamination Certificate

active

07094585

ABSTRACT:
A DNA fragment encoding a β subunit is obtained by inverse PCR using primers designed based on the nucleotide sequence of a N-terminal signal sequence region of a GDH β subunit derived fromBurkholderia cepaciaKS1 strain.

REFERENCES:
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patent: 1 331 272 (2003-07-01), None
patent: WO 02/36779 (2002-05-01), None
Inose, et al. “Cloning and Expression of the Gene Encoding Catalytic Subunit of Thermostable Glucose Dehydrogenase fromBurkholderia cepaciainEscherichia coli,” Biochmica et Biophysica Acta, 1645(2), pp. 133-138, Feb. 2003.
Sode, et al. “A Novel Thermostable Glucose Dehydrogenase Varying Temperature Properties by Altering its Quatemary Structures,”Enzyme and Microbial Technology, vol. 19, pp. 82085, 1996.
Yamazaki, et al. “Increased Thermal Stability of Glucose Dehydrogenase by Cross-Linking Chemical Modification,”Biotechnology Letters, Vo. 21, pp. 199-202, 1999.
Yamazaki, et al. “Subunit Analyses of a Novel Thermostable Glucose Dehydrogenase Showing Different Temperature Properties According to its Quaternary Structure,”Applied Biochemistry and Biotechnology, vol. 77-79, pp. 325-335, 1999.
International Search Report, issued to a related foreign application.
Supplementary European Search Report dated Oct. 19. 2005.

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