Glucose dehydrogenase

Details Glucose dehydrogenase Glucose dehydrogenase

2003-07-02

2009-06-23

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C435S183000, C435S069100, C435S320100, C435S190000, C536S023200

Reexamination Certificate

active

07550274

ABSTRACT:
Disclosed is a modified glucose dehydrogenase having pyrroloquinoline quinone as a coenzyme, wherein one or more amino acid residues in a region of 186-206 amino acid of water-soluble PQQGDH derived fromAcinetobacter calcoaceticusor in an equivalent region from other species are replaced with other amino acid residues. Also disclosed is a gene coding for the modified glucose dehydrogenase of the invention, a vector comprising the gene of the invention and a transformant comprising the vector, as well as a glucose assay kit and a glucose sensor comprising the modified glucose dehydrogenase of the invention.

REFERENCES:
patent: 2003/0232418 (2003-12-01), Takeshima et al.
patent: 1 167 519 (2002-01-01), None
patent: 1 176 202 (2002-01-01), None
patent: 1 367 120 (2003-12-01), None
patent: 2001-346587 (2001-12-01), None
patent: WO-02/34919 (2002-05-01), None
Yoshida et al., Protein Engineering, vol. 12, No. 1, pp. 63-70, (1999).
Igarashi et al., Biochemical and Biophysical Research Communication, vol. 264 pp. 820-824 (1999).
Laurinavicius et al., Talanta, vol. 52, pp. 485-493 (2000).

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