Glucose dehydrogenase

Details Glucose dehydrogenase Glucose dehydrogenase

2006-05-23

2006-05-23

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S440000, C435S004000, C435S069100, C435S183000, C435S252300, C435S320100, C536S023200, C536S023700

Reexamination Certificate

active

07049114

ABSTRACT:
Modified water-soluble glucose dehydrogenases having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved affinity for glucose.

REFERENCES:
patent: 10-243786 (1998-09-01), None
Database Swiss-Prot 'Online! Jan. 1, 1990 “Glucose dehydrogenase-B 'pyrroloquinoline-quinone! precursor” retrieved from Swiss-Prot Database accession No. P13650, XP002203013, *abstract*.
Oubrie Arthur et al.: “The 1.7 ANG crystal structure of the apo form of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat.” Journal of Molecular Biology, vol. 289, No. 2, Jun. 4, 1999, pp. 319-333, XP002202799, ISSN: 0022-2836.
Oubrie Arthur et al.: “Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: A covalent cofactor-inhibitor complex.” Proceedings of the National Academy of Sciences of the United States, vol. 96, No. 21, Oct. 12, 1999, pp. 11787-11791, XP002203011 Oct. 12, 1999, ISSN: 0027-8424.
Oubrie Arthur et al.: “Structural requirements of pyrroloquinoline quinone dependent enzymatic reactions.” Protein Science, vol. 9, No. 7, Jul. 2000, pp. 1265-1273, XP002203012, ISSN: 0961-8368.
Igarashi S. et al.: “Construction and Characterization of mutant Water-Soluble PQQ Glucose Dehydrogenases with altered Km Values Site” Biochem. Biophys. Res. Commun. (Nov., 1999) vol. 264, No. 3, pp. 820-824.
Yoshida, H. et al.: “Engineering a chimeric pyrroloquinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity” Protein Engineering (Jan., 1999) vol. 12, No. 1, pp. 63-70.
Clenton-Jansen, A. M. et al.: “Closing, characterization and DNA sequencing of the gene encoding the Mr. 50000 quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus” Mol. Genet. (1989) vol. 217, No. 2/3 pp. 430-436.

Affiliated with

Also associated with

Rating

Glucose dehydrogenase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glucose dehydrogenase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glucose dehydrogenase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3547586