Glucose and cellobiose tolerant .beta.-glucosidase from Candida

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435200, 435161, 435921, C12N 924, C12N 942, C12N 100

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active

057473200

ABSTRACT:
A new .beta.-glucosidase enzyme which is tolerant to glucose and cellobiose, and which is effective for the hydrolysis of cellobiose to glucose, is disclosed. The enzyme is produced by Candida peltata, strain NRRL Y-21603. This .beta.-glucosidase may be used in conjunction with cellulolytic enzymes for the treatment of cellulosic materials to convert cellulose to glucose.

REFERENCES:
Saha, Badal C., et al., "Thermostable .beta.-Glucosidases", Enzymatic Degradation of Insoluble Carbohydrates, Chapter 13, reprinted from ACS Symposium Series No. 618, John N. Saddler & Michael H. Penner, 1995, pp. 197-207.
Saha, B.C., and Bothast, R. J., "Glucose Tolerant and Thermophilic .beta.-Glucosidase from Yeasts", Program and Abstracts, 1995 SIM Annual Meeting, San Jose, CA, Aug. 6-11, 1995, Distributed Aug. 6, 1995.
Saha, Badal C., and Bothast, Rodney J., "Glucose Tolerant and Thermophilic .beta.-Glucosidases from Yeasts,"Biotechnology Letters, vol. 18, No. 2, Feb. 1996, pp. 155-158.
Wright, John D., "Ethanol from Biomass by Enzymatic Hydrolysis", Chemical Engineering Progress, Aug. 1988, pp. 62-74.
Ladisch, M. R., et al., "Process considerations in the enzymatic hydrolysis of biomass", Enzyme Microbiology Technology, 1983, vol. 5, Mar., pp. 82-102.
Guenata et al. J. Sci. Food Agricult. vol. 50, 1990 pp. 499-506, Abstract enclosed.
Skory et al. Appl. Microbiol. Biotechnol. 1996 vol. 46, pp. 353-359.
Wright et al. Appl. Envir. Microbiol. vol. 58, 3455-3465, 1992.

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