Gluconate dehydratase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

Reexamination Certificate

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C435S136000, C435S137000, C435S069100

Reexamination Certificate

active

07125704

ABSTRACT:
A novel gluconate dehydratase derived fromAchromobacter xylosoxidansand a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.

REFERENCES:
patent: 6562958 (2003-05-01), Breton et al.
Kersters et al (and properties of D-gluconate dehydratase from Achromobacter. Antonie Van Leewenhoek 37 (1971) 233-246.
R. Bender et al., “Enzymatic Synthesis of 2-Keto-3-deoxy-D-gluconate from D-Gluconate,”Analytical Biochemistry, 1974, pp. 275-279, vol. 61, Academic Press, Inc.
K. Kersters et al., “2-Keto-3-deoxy-D-gluconate,”Methods of Enzymology, 1975, pp. 99-101, vol. 41.
B. Nicolaus et al., “Production of 2-Keto-3-Deoxygluconate by Immobilized Cells of Sulfolobus Solfataricus,”Biotechnology Letters, 1986, pp. 497-500, vol. 8, No. 7.

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