Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1987-04-27
1988-12-27
Martinell, James
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 78, 435 91, 4351721, 4351723, 435205, 435254, 435320, 435 14, 435 19, 435 21, 435 29, 435 68, 435 72, 435 73, C07H 2104, C12P 2102, C12P 1934, C12N 1500, C12N 934, C12N 120, C12N 114
Patent
active
047941758
ABSTRACT:
A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly A RNA obtained from fungal cells grown under conditions of glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host is preferably bacteria or yeast. The transformed yeast host may be used to produce ethanol.
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Flatgaard Jeffrey E.
Innis Michael A.
Nunberg Jack
Cetus Corporation
Halluin Albert P.
Hasak Janet E.
Martinell James
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