Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-10-16
1999-10-12
Caputa, Anthony C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435975, 5303882, 53038825, 5303887, G01N 3353, G01N 33567, C07K 1600, C12P 2108
Patent
active
059653744
ABSTRACT:
The present invention is directed to monoclonal antibodies which specifically bind to a Limulus .alpha..sub.2 -macroglobulin. Preferred monoclonal antibodies inhibit the enzymatic activation of a Limulus amebocyte lysate to form a gel or to cleave a chromogenic substrate in the presence of an endotoxin but not in the presence of a glucan. The monoclonal antibodies can be used, inter alia, to purify a Limulus .alpha..sub.2 -macroglobulin, and the preferred antibodies can be used in a method for specifically detecting a glucan in a test sample using an amebocyte lysate.
REFERENCES:
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Herbstritt Christopher J.
Pepe Michael G.
BioWhittaker Technologies, Inc.
Caputa Anthony C.
Navarro Mark
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