Glucagon antagonists

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Glucagon; related peptides

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530324, A61K 3826, C07K 1400, C07K 14605

Patent

active

055104592

ABSTRACT:
Methods for detecting glucagon antagonists through the use of recombinant DNA techniques are provided. Briefly, subsequent to the expression of glucagon analogs within suitable host cells, the analogs are exposed to a glucagon receptor coupled to a response pathway in the presence of native glucagon. A reduction in the stimulation of the response pathway resulting from the binding of the glucagon analog to the glucagon receptor relative to the stimulation of the response pathway by native glucagon alone indicates the presence of a glucagon antagonist. Glucagon antagonists identified and isolated through the methods are also provided.

REFERENCES:
Zurawski et al. EMBO vol. 8(9) pp. 2583-2590 (1989).
Gysin et al. Biochem. vol. 25 pp. 8278-8284 (1986).
Sueiras-Diaz et al., "Structure-Activity Studies on the N-Terminal Region of Glucagon", J. Med. Chem. 27:310-315, 1984.
P. Robberecht et al., "Comparative Efficacy of Seven Synthetic Glucagon Analogs, Modified in Postions 1, 2 and/or 12, on Liver and Heart Adenylate Cyclase from Rat", Chemical Abstracts vol. 105, No. 17, Oct. 27, 1986 (Abstract No. 146405).
R. Merrifield et al., "Synthetic Peptide Antagonists of Glucagon", Chemical Abstracts vol. 108, No. 9, Feb. 29, 1988 (Abstract No. 69120).
Unson et al., "Glucagon Antagonists: Contribution to Binding and Activity of the Amino-terminal Sequence 1-5, Position 12 and the Putative .alpha.-Helical Segment 19-27", The Journal of Biological Chemistry 264:789-794, 1989.
Unson et al., "Specificity of Position 9 for Glucagon Activity", Peptides 729-731, 1991.
Gysin et al., "Design and Synthesis of Glucagon Partial Agonists and Antagonists", Biochemistry 25:8278-8284, 1986.
Unson et al., "Postion 9 Replacement Analogs of Glucagon Uncouple Biological Activity and Receptor Binding", The Journal of Biological Chemistry 266:2763-2766, 1991.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Glucagon antagonists does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glucagon antagonists, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glucagon antagonists will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2309451

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.