Glia activating factor and its production

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 231, 536 235, 435 711, 4351721, 4352401, 4352523, 43525411, C12N 1512, C12N 121, C12N 510, C12P 2106

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055124607

ABSTRACT:
Disclosed are (1) a novel glia activating factor which has glial cell growth promoting activity; (2) a glia activating factor containing a polypeptide represented by SEQ ID NO: 1 or a mutein thereof having activity of the factor; (3) a DNA containing a polynucleotide coding for a glia activating factor represented by SEQ ID NO: 10; (4) a host transformed with a vector containing the DNA described in (3); and (5) a method for producing the above glia activating factor which comprises cultivating the transformant described in (4) in a medium accumulating the glia activating factor in a culture broth, and collecting the resulting glia activating factor.

REFERENCES:
Kimura et al. letter "Structure, expression and function of a schwannoma-derived growth factor", Nature, vol. 348, pp. 257-260 (1990).
Guenther et al. article "A glia-derived neurite-promoting factor with protease inhibitory activity", The EMBO Journal, vol. 4, No. 8, pp. 1963-1966 (1985).
Giulian et al., article "Brain peptides and Glial Growth: II. Identification of Cells That Secrete Glia-Promoting Factors", The Journal of Cell Biology, vol. 102, pp. 812-829, Mar. 1986.
Giulian et al., article "Brain peptides and Glial Growth: I. Glia-Promoting Factors as Regulators of Gligoeneis in the Developing and Injured Nervous System", The Journal of Cell Biology, vol. 102, pp. 803-811, Mar. 1986.
Chemical Abstracts, vol. 98, No. 15, Apr. 11, 1983, Columbus, Ohio, US; Abstract No. 123664A, Nister M. et al: "A platelet-derived growth factor analog produced by a human clonal glioma cell line", p. 445; column L.
Chemical Abstracts, vol. 96, No. 1, Jan. 4, 1982, Columbus, Ohio, US; Abstract No. 4542H, Kato, Taiji et al.: "Glial cell growth-promoting factor in astrocytoma C6 cell extracts", p. 421; column L.
Chemical Abstracts, vol. 110, No. 11, Mar. 13, 1989, Columbus, Ohio, US; Abstract No. 89388T, Guilan, Dana et al.: "Secreted peptides as regulators of neuron-glia and glia--glia interactions in the developing nervous system" p. 162; column L.
The Journal of Neuroscience, vol. 4, No. 1, Jan. 1984, Washington, D.C.; USA pp. 75-83; Greg Erwin Lemke et al: "Identification and purification of glial growth factor".

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