Glassified restriction enzymes

Details Glassified restriction enzymes Glassified restriction enzymes

1991-09-20

1993-10-05

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 18, 435 19, 435178, 435183, 435187, 435188, 435195, C12N 914, C12N 916, C12Q 134, C12Q 144

Patent

active

052504298

ABSTRACT:
A room temperature stable restriction enzyme in a glassified carbohydrate stabilizer is disclosed. A restriction enzyme reaction buffer containing Mg.sup.+2 is dispersed in the stabilizer. One cleaves DNA merely by adding water and DNA to the composition. The composition can reduce glycerol-caused star activity by avoiding the need for glycerol. The composition also reduces star activity caused by high concentrations of enzymes. Another method is provided of comparing cleaved DNA samples in connection with forensic and paternity testing applications.

REFERENCES:
patent: 3300474 (1967-01-01), Flodin et al.
patent: 5098893 (1992-03-01), Franks et al.
10 pages Pharmacia article entitled "Ficoll for cell research", undated admitted prior art.
Pp. 5.3-5.32 of J. Sambrook et al., A Laboratory Manual, (1989).
J. George et al., 79 P.N.A.S. USA 2432-36 (1982).
W. Malyguine et al., 8 Gene 163-177 (1980).

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