Glass-surface microcarrier for growth of cell cultures

Details Glass-surface microcarrier for growth of cell cultures Glass-surface microcarrier for growth of cell cultures

1982-03-03

1984-05-15

Naff, David M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435176, 435240, 502439, 502159, C12N 500, C12N 502, C12N 1114

Patent

active

044488840

ABSTRACT:
A microcarrier for anchorage-dependent cell cultivation is prepared containing a spherical substrate of polymeric material having a bulk density of about 1 g/cc so as to be substantially buoyant in an aqueous culture medium, and a thin (less than 1 .mu.m) coating layer of silicate glass. The silicate glass coating layer is preferably applied to a spherical precursor of polymeric material in an rf sputtering operation. An intermediate coating layer of magnetic material may be deposited prior to the silicate glass layer, so that the microcarriers may be readily removed from culture media by suitable subjection to a magnetic field.

REFERENCES:
patent: 3717551 (1973-02-01), Bizzini et al.
patent: 4036693 (1977-07-01), Levine et al.
patent: 4177253 (1979-12-01), Davies et al.
patent: 4189534 (1980-02-01), Levine et al.
patent: 4266032 (1981-05-01), Miller et al.
Maissel et al., Handbook of Thin Film Technology, McGraw-Hill Book Co., NY, 1970, (pp.1-44).
"Cytodex.TM.", Beaded Microcarriers for Cell Culture, Pharmacia Fine Chemicals, Uppsala, Sweden, 1978, (p. 6).

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