Gibberellin 3β-hydroxylase genes of rice and uses thereof

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C536S023200, C536S023600, C435S320100, C435S419000, C435S195000, C800S278000

Reexamination Certificate

active

07049490

ABSTRACT:
Genomic DNA and cDNA encoding GA 3β-hydroxylase were isolated from rice. When the expression of these genes was suppressed in rice plants, the plants became dwarfed compared with the wild type plants.

REFERENCES:
patent: 5925807 (1999-07-01), Chiang et al.
patent: 5939539 (1999-08-01), Lange et al.
patent: WO 98/59057 (1998-12-01), None
patent: WO 01/48215 (2001-07-01), None
Martin, D.N. et al. “Mendel's dwarfing gene: cDNAs from theLealleles and function of expressed proteins”Proc. Natl. Acad. Sci. USA94:8907-8911 (Aug. 1997).
Chiang, H-H. et al. “Isolation of the arabidopsis GA4 locus”The Plant Cell7:195-201 (Feb. 1995).
Lange, T. et al. “Expression cloning of a gibberellin 20-oxidase, a multifunction exzyme involved in gibberellin biosynthesis”Proc. Natl. Acad. Sci. USA91:8552-8556 (Aug. 1994).
Lester, D.R. et al. “Mendel's stem length gene (Le) encodes a gibberellin 3β-hydroxylase”The Plant Cell9:1435-1443 (Aug. 1997).
Itoh, H. et al. “Cloning and functional analysis of two gibberellin 3β-hydroxylase genes that are differently expressed during the growth of rice”Proc. Natl. Acad. Sci. USA98(15):8908-8914 (Jul. 17, 2001).
Lange, T. et al. “Cloning and expression of a gibberellin 2β, 3β-hydroxylase cDNA from pumpkin endosperm”Plant Cell9(8):1459-67 (Aug. 1997).
Sakamoto, T. et al. “Gibberellin seigousei kouso idenshi no hatsugen chousetsu ni yoru ine kusagata no jiniteki seigyo”Kagaku to Seibutsu38(2):131-139 (Feb. 2000).
Hedden, P. et al. “Recent advances in gibberellin biosynthesis”Journal of Experimental Biology50(334):553-563 (May 1999).
Yamaguchi, S. et al. “Gibberellin no seigousei kenkyu; saishin no shinpo(1)”Kagaku to Seibutsu34(6):402-410 (Jun. 1996).
Sakamoto et al. “Artificial regulation of rice plant shape by controlling the expression of gibberellin biosynthetic enzyme gene”Kagaku to Seibutsu38(2):131-139 (2000).
Yamaguchi et al. “Recent progess in studies of gibberellin biosynthesis”Kagaku to Seibutsu34(6):402-410 (1996).
Honda, Ichiro, et al., “Characterization of Endogenous Gibberellins in Dwarf Rice Mutants,”Biosci. Biotech. Biochem.,vol. 60(12):2073-2075 (1996).

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