Gibberellin 2-oxidase

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S290000, C435S419000

Reexamination Certificate

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10670454

ABSTRACT:
A nucleic acid sequence is provided which encodes a gibberelin 2-oxidase gene which catalyzes the 2β-oxidation of a gibberelin molecule to introduce a hydroxyl group at C-2 and further catalyzes the oxidation of the hydroxyl group introduced at C-2 to yield the ketone derivative. Such sequences can find application in the preparation of transgenic plants with altered levels of gibberelin 2-oxidase.

REFERENCES:
patent: 9000069 (1997-01-01), None
Thomas et al (1999, PNAS 96:4698-4703).
Biemelt et al (2004, Plant Physiology 135:254-265).
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Derwent World Patents Index, Accession No. 1997-112670, English language abstract for JP 9-000069 (Document AL1).
EMBL Sequence Database, EMBL amino acid sequence from MacMillan, J., et al., “Gibberellin biosynthesis from gibberellin Al2-aldehyde in endosperm and embryos ofMarah macrocarpa,” Plant Physiol.113:1369-1377 (Apr. 1997), EMBL Accession No. 004162 (submitted Jul. 1, 1997).
EMBL Sequence Database, EMBL amino acid sequence from MacMillan, J., et al., “Gibberellin biosynthesis from gibberellin Al2-aldehyde in endosperm and embryos ofMarah macrocarpa,” Plant Physiol.113:1369-1377 (Apr. 1997), EMBL Accession No. Y09113 (submitted Nov. 5, 1996).
Hedden, P., and Phillips, A.L., “Gibberellin metabolism: new insights revealed by the genes,”Trends Plant Sci.5:523-530, Elsevier Science Ltd (Dec. 2000).
MacMillan, J., et al., “Gibberellin Biosynthesis from Gibberellin A12-Aldehyde in Endosperm and Embryos ofMarah macrocarpus,” Plant Physiol.113:1369-1377, American Society of Plant Physiologists (Apr. 1997).
McConnell, J.R., et al., “Role ofPHABULOSAandPHAVOLUTAin determining radial patterning in shoots,”Nature 411:709-713, Macmillan Magazines Ltd (Jun. 2001).
Sakamoto, T., et al., “Expression of a Gibberellin 2-Oxidase Gene Around the Shoot Apex Is Related to Phase Transition in Rice,”Plant Physiol.25:1508-1516, American Society of Plant Physiologists (Mar. 2001).
Singh, D.P., et al., “Gibberellins Are Required for Seed Development and Pollen Tube Growth inArabidopsis,” Plant Cell 14:3133-3147, American Society of Plant Biologists (Dec. 2002).
Thomas, S.G., et al., “Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation,”Proc. Natl. Acad. Sci. USA 96:4698-4703, National Academy of Sciences (Apr. 1999).
Hedden, P., “Constructing dwarf rice,”Nat. Biotechnol.21:873-874, Nature Publishing Co. (Aug. 2003).
Sakamoto, T., et al., “Genetic manipulation of gibberellin metabolism in transgenic rice,”Nat. Biotechnol.21:909-913, Nature Publishing Co. (Aug. 2003).

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