GFP fusion proteins and their use

Details GFP fusion proteins and their use GFP fusion proteins and their use

2002-07-03

2011-11-22

Steadman, David J (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S194000, C435S007100, C435S007600, C530S350000, C530S387300

Reexamination Certificate

active

08062874

ABSTRACT:
The present invention provides fusion proteins including a green fluorescent protein inserted into the internal amino acid sequence of a Gαs protein and further provides method of using the fusion protein construct to follow activation of a G-protein receptor by a candidate drug.

REFERENCES:
patent: 5625048 (1997-04-01), Tsien et al.
patent: 5683888 (1997-11-01), Campbell et al.
patent: 5976796 (1999-11-01), Szalay et al.
patent: 9814605 (1998-04-01), None
patent: 9821355 (1998-05-01), None
patent: 9836081 (1998-08-01), None
Ngo et al., In the Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston; MA, pp. 433 and 492-495.
Hughes et al., Journal of Biological Chemistry, 2001, vol. 276(6): 4227-4235.
Yuan et al., Journal of Biological Chemistry, 2000, 275(3): 2157-2164.
Milligan et al., Heterotrimeric G-proteins: a short history, British Journal of Pharmacology, 2006, 147: S46-S55.
Meszaros et al., Identification of G protein-coupled signaling pathways in cardiac fibroblasts: cross talk between Gq and Gs, Am. J. Physiol. Cell Physiol., 2000, 278: C154-C162.
Levis et al., Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity, Journal of Cell Biology, 1992, vol. 119, No. 5, pp. 1297-1307.
Liu et al., The helical domain of a G protein subunit is a regulator of its effector, Proc. Natl. Acad. Sci., 1998, vol. 95, pp. 12878-12883.
Blast 2 Sequences, sequence alignment results. Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi>, last viewed on Mar. 24, 2008.
Antonelli et al. (Human-Xenopus chimeras of Gs reveal a new region important for its activation of adenylyl cyclase, FEBS Letters, 340 (1994), pp. 249-254).
Yu, Y.Z. (2002) “Real-timeVisualization of a Fluorescent Galphas: Dissociation of the Activated G Protein from plasma Membrane.”Molecular Pharmacology. vol. 61. No. 2: 352-359.
Goto, Katsutoshi, et. al. (2000) “Subtype-Specific Trafficking of Endothelin Receptors.”Journal of Biological Chemistry. vol. 275. No. 12: 8664-8671.
Kitamura, Toshio, et. al. (2000) “A Method to Identify cDNAs based on localization of Green Fluorescent Protein Fusion Products.”Proceedings of the National Academy of Sciences of the United States of America. vol. 97. No. 7: 3062-3066.
Siegel, M.S., et. al. (1997) “A Genetically Encoded Optical Probe of Membrane Voltage.”Neuron. vol. 19. No. 4: 735-741.
Liu, J., et. al. (1997) “The First 35 Amino Acids and Fatty Acylation Sites Determine the Molecular Targeting of Endothelial Nitric Oxide Synthase into the Golgi Region of Cells: A Green Fluorescent Protein Study.”Journal of Cell Biology. vol. 137. No. 7: 1525-1535.
Biondi, R.M., et. al. (1998) “Random Insertion of GFP into the cAMP-Dependent Protein Kinase Regulatory Subunit fromDictyostelium discoideum.”Nucleic Acids Research. vol. 26. No. 21: 4946-4952.
Kudo, N., et. al. (1997) “Molecular Cloning and Cell Cycle-Dependent Expression of Mammalian CRM1, a Protein Involved in Nuclear Export of Proteins.”J. Biol. Chem. vol. 272. No. 47: 29742-29751.
Romoser, V.A., et. al. (1997) “Detection in Living Cells of Ca Super (2+)-Dependent Changes in the Fluorescence Emission of an Indicator Composed of Two Green Fluorescent Protein Variants Linked by a Calmodulin-Binding Sequence. A New Class of Fluorescent Indicators.”J. Biol. Chem. vol. 272. No. 20: 13270-13274.

Affiliated with

Also associated with

Rating

GFP fusion proteins and their use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with GFP fusion proteins and their use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and GFP fusion proteins and their use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4294729