Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2007-06-05
2007-06-05
Kaushal, Sumesh (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S003000, C800S025000
Reexamination Certificate
active
10473637
ABSTRACT:
A system for observing the behaviors of mitochondria during ontogeny and the dynamics of mitochondria in various tissues during pathological development is provided. A pCAGGS expression vector containing green fluorescent protein (GFP) gene and a transgenic animal characterized by expressing GFP specifically in mitochondria are provided.
REFERENCES:
patent: 2001-309736 (2001-11-01), None
Rizzuto R, Chimeric green flourescent protein as a tool for visualizing subcellular organelles in living cells, 1005, Current Biology, vol. 5, pp. 635-642.
Olson M, Mitochondria in apopotsis and human disease, 2001, Current Molecular Medicine, vol. 1, pp. 91-122. □□
Yano M, Visualization of mitochondrial protein import in cultured mammalian cells with green floursecent protein and effects of overexpression of the human import receptor Tom20, JBC, 1997, vol. 272, pp. 8459-8465.
Sun K, Role of mitochondria in cell apoptosis during hepatic ischemia-repurfusion injury and protective effect of ischemic postconditioning, 2004, World J Gastoenterol, vol. 10, pp. 1934-1938.
Arden N, Life and death in mammalian cell culture: strategies for apoptosis inhibition, 2004, TIBS, vol. 22, pp. 174-180.
Shitara H, Non-invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green flourescent protein (GFP), 2001, FEBS, vol. 500, pp. 7-11.
Ristevski S, Making better transgenic models, 2005, Molecular Biotechnology, vol. 29, pp. 153-163.
Smith KR, Gene transfer in higher animals: theoretical considerations and key concepts, J. of Biotechnology, vol. 99, pp. 1-22.
Houdebine LM, The methods to generate transgenic animals and to control transgene expression, 2002, J. of Biotechnology, vol. 98, pp. 145-160.□□
Shitara et al., Generation and Application of Transgenic Mouse Having Mitochondria Visualized by Green Flourescent Protein Labeling, 72ndAnnual Meeting of the Genetic Society of Japan, Program and Collection of Preliminary Reports, Sep. 30, 2000.
Shitara, H., et al., “Non-invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green flourescent protein (GFP)”, FEBS Letters, (Jun. 2001), vol. 500, No. 1-2, pp. 7-11.
Rizzuto, R., et al., “Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo”, Curr. Biol., (1998), vol. 6, No. 2, pp. 183-188.
Rizzuto, R., et al., “Chimeric green flourescent protein as a tool for visualizing subcellular organelles in living cells”, Curr. Biol., (1995), vol. 5, No. 6, pp. 635-642.
Niwa, H., et al., “Efficient selection for high-expression transfectants with a novel eukaryotic vector”, Gene, (1991), vol. 108, No. 2, pp. 193-199.
Ikawa, Masato, et al., “Hikaru Mouse!? Hakkou Owan Kurage no Idenshi wo Mouse de Hatsugen; Kouhan na Ouyou ni Kitai”, Kagaku to Seibutsu, (1998), vol. 36, No. 3, pp. 140-141 (and English Translation).
Matsuyama, Shigemi, “Saibou-nai pH Henka to Apoptosis”, Jikken Igaku, (2000), vol. 18, No. 14, pp. 1923-1926 (and English translation of the relevant parts thereof).
Barr, P.J., et al., “Apoptosis and Its Role in Human Disease”, Bio/Technology, vol. 12, pp. 487-483, May 12, 1994.
Yuan, J., et al., “Apoptosis in the Nervous System”, Nature, vol. 407, pp. 802-809, Oct. 12, 2000.
Gao, W., et al., “Temporal Relationship Between Cytochrome C Release and Mitochondrial Swelling During UV-Induced Apoptosis in Living HeLa Cells”, Journal of Cell Science, vol. 114, pp. 2855-2862, May 2, 2001.
Frank, S., et al., “The Role of Dyamin-Related Protein 1, a Mediator of Mitochondrial Fission, in Apoptosis”, Developmental Cell, vol. 1, pp. 515-525, Oct. 2001.
Miyazaki Jun-ichi
Shitara Hiroshi
Yonekawa Hiromichi
Davidson Davidson & Kappel LLC
Kaushal Sumesh
Montanari David
Tokyo Metropolitan Organization for Medical Research
LandOfFree
GFP expression vector localized in mitochondria does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with GFP expression vector localized in mitochondria, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and GFP expression vector localized in mitochondria will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3836121