Germ cell alkaline phosphatase

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 8 to 10 amino acid residues in defined sequence

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Details

530300, 530329, 530350, 530403, 530806, 435 21, 435195, C07K 706, C07K 710

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active

050812270

ABSTRACT:
The invention relates to structural and regulatory DNA sequences encoding the germ cell ALP gene. These sequences differ at identified positions from the PLAP gene. Labelled nucleotide sequences complementary to germ cell ALP encoding nucleotide sequences but differing from PLAP may be used to detect the presence of the gene. The invention also relates to gene fragments specifying amino acid sequences which are specific to germ cell ALP and to antibodies raised against germ cell ALP-specific peptide fragments, their diagnostic and therapeutic uses.

REFERENCES:
patent: 4375514 (1983-03-01), Siewert et al.
Watanabe et al., J. of Biol. Chem., vol. 264, No. 21, Jul. 25, 1989, pp. 12611-12619.
Millan et al., Proc. Nat. Acad. Sci, vol. 85, May 1988 (Developmental Biology), pp. 3024-3028.
Millan, Jose; Nustad, Kjell; Norgaard-Pedersen, 1985, Highly Sensitive Solid-Phase Immunoenzymometric Assay for Placental and Placental-Like Alkaline Phosphatases with a Monoclonal Antibody and Monodisperse Polymer Particles, Clinical Chemistry, 31:1:54-59.
Millan, Jose; Stigbrand, Torgny, 1983, Antigenic Determinants of Human Placental and Testicular Placental-Like Alkaline Phosphatases as Mapped by Monoclonal Antibodies, Eur. J. Biochem. 136: 1-7.
Shameem, G. et al., Clin. Chem. 33, No. 2: 248-252 (1987).
Wei, S. et al., Eur. J. Biochem., 118:39-45 (1981).
Millan, Jose, Jour. Biol. Chem., 261, No. 7:3112-3115 (1986).

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