Geranylgeraniol-18-hydroxylase from croton sublyratus

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435157, 435189, C12P 702, C12P 704, C12N 902

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058799168

ABSTRACT:
Geranylgeraniol-18-hydroxylase, which catalyzes the C-18 hydroxylation of geranylgeraniol to form plaunotol, is extracted from Croton sublyratus using an extraction buffer and is purified using a differential centrifugation separation technique. The purified enzyme is used to convert geranylgeraniol to plaunotol, an anti-peptic ulcer agent. The enzymatic activity of the hydroxylase can be increased by a factor of about three by heating the enzyme in boiling water for about 30 minutes. The enzyme activity is also increased by adding a coenzyme, NADPH, which increases enzyme activity by a factor of about 2 or NADH, which increases activity by a factor of about 1.4. The pH optimum for hydroxylase enzyme activity is 5.0 The hydroxylase is a complex enzyme with a diameter of about 20 to 40 nm.

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